Development of a UPLC-TQ/MS Approach for the Determination of Eleven Bioactive Components in Haizao Yuhu Decoction Plus-Minus Haizao and Gancao Drug Combination after Oral Administration in a Rat Model of Hypothyroidism.

Abstract	Haizao Yuhu Decoction (HYD) has been used for approximately 500 years and is well-known in Traditional Chinese Medicine for its efficacy in the treatment of thyroid-related diseases. In this study, a rapid liquid chromatography-tandem mass spectrometry method was developed for the determination of liquiritin, naringin, hesperidin, peimine, liquiritigenin, glycyrrhizic acid, bergapten, nobiletin, osthole, and glycyrrhetinic acid in rat plasma to investigate the pharmacokinetic profile of different HYD prescriptions in a rat model of hypothyroidism. The differences in pharmacokinetic parameters among the groups were compared by Student's t-test. The pharmacokinetic profile of liquiritin, naringin, hesperidin, peimine, liquiritigenin, glycyrrhizic acid, bergapten, nobiletin, osthole, and glycyrrhetinic acid showed significant differences between Haizao and Gancao anti-drug combination and other herbs in HYD. These results may contribute to the rational clinical use of HYD and reveal the compatibility profile of the Haizao and Gancao anti-drug combination.
Authors	Yingchang Ma, Yang Zhang, Yuanjuan Zhai, Zhenhua Zhu, Ying Pan, Dawei Qian, Shulan Su, Xinsheng Fan, Jinao Duan
Journal	Molecules (Basel, Switzerland) (Molecules) Vol. 22 Issue 1 (Dec 22 2016) ISSN: 1420-3049 [Electronic] Switzerland
PMID	28025523 (Publication Type: Journal Article)
Chemical References	Coumarins Drugs, Chinese Herbal Flavanones Immunologic Factors Pentacyclic Triterpenes haizao yuhu
Topics	Administration, Oral Animals Chromatography, High Pressure Liquid (methods) Coumarins (blood, pharmacokinetics) Disease Models, Animal Drugs, Chinese Herbal (pharmacokinetics, pharmacology) Flavanones (blood, pharmacokinetics, pharmacology) Humans Hypothyroidism (drug therapy, immunology, pathology) Immunologic Factors (blood, pharmacokinetics) Limit of Detection Male Medicine, Chinese Traditional Pentacyclic Triterpenes (blood, pharmacokinetics) Rats Rats, Wistar Reproducibility of Results Thyroid Gland (drug effects, immunology, pathology)

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