Isoflavone genistein inhibits estrogen-induced chloride and bicarbonate secretory mechanisms in the uterus in rats.

Abstract

We hypothesized that genistein could affect the chloride (Cl- ) and bicarbonate (HCO3- ) secretory mechanisms in uterus. Ovariectomized female rats were given estradiol or estradiol plus progesterone with 25, 50, or 100 mg/kg/day genistein. Following completion of the treatment, uterine fluid Cl- and HCO3- concentrations were determined by in vivo uterine perfusion. Uteri were subjected for molecular biological analysis (Western blot, qPCR, and immunohistochemistry) to detect levels of expression of Cystic Fibrosis transmembrane regulator (CFTR), Cl- /HCO3- exchanger (SLC26a6), Na+ /HCO3- cotransporter (SLC4a4), and estrogen receptor (ER)-α and β. Coadministration of genistein resulted in decrease in Cl- and HCO3- concentrations and expression of CFTR, SLC26a6, SLC4a4, and ER-α and ER-β in the uteri of estradiol-treated rats. In estradiol plus progesterone-treated rats, a significant increase in the above parameters were observed following high-dose genistein treatment except for the SLC24a4 level. In conclusion, genistein-induced changes in the uterus could affect the reproductive processes that might result in infertility.

• Authors: Asma Chinigarzadeh, Kamarulzaman Karim, Sekaran Muniandy, Naguib Salleh
• Journal: Journal of biochemical and molecular toxicology (J Biochem Mol Toxicol) Vol. 31 Issue 4 (Apr 2017) ISSN: 1099-0461 [Electronic] United States
• PMID: 27891704 (Publication Type: Journal Article)
• Copyright: © 2016 Wiley Periodicals, Inc.

Chemical References

• Antiporters
• Bicarbonates
• CFTR protein, rat
• Chlorides
• Estrogens
• Receptors, Estrogen
• SLC26A6 protein, rat
• Slc4a4 protein, rat
• Sodium-Bicarbonate Symporters
• Sulfate Transporters
• Cystic Fibrosis Transmembrane Conductance Regulator
• Genistein

Topics

• Animals
• Antiporters (drug effects, genetics)
• Bicarbonates (metabolism)
• Chlorides (metabolism)
• Cystic Fibrosis Transmembrane Conductance Regulator (drug effects, genetics)
• Estrogens (pharmacology)
• Female
• Gene Expression Regulation
• Genistein (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Receptors, Estrogen (drug effects, genetics)
• Sodium-Bicarbonate Symporters (drug effects, genetics)
• Sulfate Transporters
• Uterus (drug effects, metabolism)