The Transient Receptor Potential Vanilloid type-1 (TRPV1) channel is a non-selective
cation channel belonging to the Transient Receptor Potential family; variation of its expression has been correlated to
glioma progression. In human, TRPV1 transcripts display a remarkable homogeneity differing only for the 5'-untranslated region (
5'UTR) sequence that generates four variants encoding the same
protein. Herein, we investigated the role of the
5'UTR sequences in TRPV1 transcripts stability, regulation of translation, expression in
glioma cells and tissues. In addition, the expression of
5'UTR TRPV1 variants as prognostic factor in the survival of
glioblastoma patients was evaluated. The expression level for each
5'UTR and their stability was evaluated by RT-PCR analysis. The effect of
rapamycin and
interferon-gamma in 5'UTR-regulating TRPV1 translation was determined by western blot analysis in
glioma cell lines. We demonstrated that the
5'UTR influences the stability and translation efficacy of TRPV1 transcripts, and that TRPV1 variant three (TRPV1v3) was the most stable and the only variant expressed in GBM samples and in
glioma stem-like cells. Furthermore, we found that TRPV1v3 expression levels correlate with patient's survival, suggesting that it may represent a potential prognostic marker for patients with
glioma.