Humoral and Cellular Responses to a Single Dose of Fendrix in Renal Transplant Recipients with Non-response to Previous Hepatitis B Vaccination.

Abstract

Approximately 70% of kidney transplant recipients are non-responders to conventional hepatitis B virus (HBV) vaccines. We examined whether Fendrix™, an HBV vaccine containing 3-O-desacyl-4'-monophosphoryl lipid A (MPL) as adjuvant, could induce HBV immunity in these patients and compared their vaccination efficacy with healthy controls tested previously by the same assays. We selected 35 kidney transplant recipients who had been vaccinated at least thrice against HBV but had never displayed anti-HBs antibodies. We re-assessed their anti-HBs antibody titres and further determined cellular HBV immunity by proliferation assay and interferon (IFN)-γ ELISpot. Seventeen recipients did neither display humoral nor cellular immunity and could be tested prior to and at month 1 after vaccination. Of note, HLA antigens associated with non-response to HBV vaccination (HLA-DRB1*03 and HLA-DQB1*02) were over-represented in these 17 recipients. At month 1 after a single vaccination with Fendrix™, we observed a significant increase in anti-HBs antibodies (P = 0.02). In seven of 17 recipients, we detected anti-HBs antibodies ≥10 IU/l (10-264), in four HBV-specific lymphocyte proliferation (stimulation index of 2.6-8.7) and in one specific IFN-γ responses (12 spots increment). The vaccination response to Fendrix™ was significantly higher (P = 0.035) than the response to HBVaxPro™ in young healthy controls. In summary, the results show that a single vaccination with Fendrix™ could already induce HBV-specific humoral and/or cellular responses in ten of 17 kidney transplant patients. Thus, Fendrix™ appears as an efficient vaccine in this patient cohort.

• Authors: M Lindemann, M Zaslavskaya, M Fiedler, B Wilde, F M Heinemann, A Heinold, P A Horn, O Witzke
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 85 Issue 1 Pg. 51-57 (Jan 2017) ISSN: 1365-3083 [Electronic] England
• PMID: 27763680 (Publication Type: Journal Article)
• Copyright: © 2016 The Foundation for the Scandinavian Journal of Immunology.

Chemical References

• Antibodies, Viral
• Fendrix
• Hepatitis B Antibodies
• Hepatitis B Vaccines
• Interferon-gamma

Topics

• Adult
• Aged
• Antibodies, Viral (blood)
• Cell Proliferation
• Cells, Cultured
• Enzyme-Linked Immunospot Assay
• Female
• Hepatitis B (immunology, prevention & control)
• Hepatitis B Antibodies (immunology)
• Hepatitis B Vaccines (immunology)
• Humans
• Immune Tolerance
• Immunity, Cellular
• Immunity, Humoral
• Interferon-gamma (metabolism)
• Kidney Transplantation
• Male
• Vaccination
• Young Adult