OTUB1 attenuates neuronal apoptosis after intracerebral hemorrhage.

Abstract

OTUB1 is a member of deubiquitinating enzymes, which was shown as a proteasome-associated DUB to be involved in the proteins Ub-dependent degradation. Previous studies have indicated that OTUB1 was expressed in brain. But its distribution and function in the brain remain unclear. In this study, we explored the roles of OTUB1 protein in the pathophysiology of intracerebral hemorrhage (ICH). From the results of Western blot, immunohistochemistry, and immunofluorescence, we found an obvious up-regulation of OTUB1 in neurons adjacent to the hematoma after ICH. Furthermore, we also found that the increase of OTUB1 expression was accompanied by the enhanced expression of Bax and active caspase-3, and decreased expression of Bcl-2 in the pathological process of rat ICH. What's more, our in vitro study, using OTUB1 RNA interference in PC12 cells, suggested that OTUB1 might exert its anti-apoptotic function in neuronal apoptosis. Therefore, OTUB1 may play a role in protecting the brain from secondary damage following ICH.

• Authors: Lili Xie, Aihong Li, Jiabing Shen, Maohong Cao, Xiaojin Ning, Debin Yuan, Yuteng Ji, Hongmei Wang, Kaifu Ke
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 422 Issue 1-2 Pg. 171-180 (Nov 2016) ISSN: 1573-4919 [Electronic] Netherlands
• PMID: 27629786 (Publication Type: Journal Article)

Chemical References

• Endopeptidases
• OTUB1 protein, rat

Topics

• Animals
• Apoptosis
• Cerebral Hemorrhage (enzymology, pathology)
• Endopeptidases (biosynthesis)
• Gene Expression Regulation, Enzymologic
• Male
• Neurons (enzymology, pathology)
• PC12 Cells
• Rats
• Rats, Sprague-Dawley