p53 and ki67 as biomarkers in determining response to chemoprevention for oral leukoplakia.

Abstract

BACKGROUND:

We performed a randomized controlled chemoprevention trial of oral leukoplakia by administrating a low dose of beta-carotene and vitamin C supplements. 17% of subjects in the experimental arm (4/23) demonstrated clinical remission (complete or partial response) at completion of the trial. The objective of this study was to determine whether baseline expression of p53 and ki67 demonstrated any differences between those responding or not responding to our intervention. A secondary objective was to elucidate any relationship between dietary factors and clinical responses.

METHODS:

For this biomarker study, we included all subjects in the experimental group (n = 23) who were non-smokers. Among 16 who completed the trial for 1 year of supplementation, there were four responders and 12 non-responders at 1-year follow-up. Following immuno-staining for p53 and ki67, the percentage of positive cell nuclei were analyzed as labeling index (LI).

RESULTS:

Expression of p53 was greater in basal layers than in para-basal layers. Mean para-basal LI of p53 was higher in non-responding (26.0) than in responding subjects (11.2) (P = 0.028). ki67 LIs were not significantly different in the two groups.

CONCLUSIONS:

Expression of p53 was inversely related to clinical response to the supplements. Other biomarkers that may recognize subject's responsiveness to chemoprevention require further study.

• Authors: Toru Nagao, Saman Warnakulasuriya, Hidenori Sakuma, Satoru Miyabe, Shogo Hasegawa, Junichiro Machida, Koji Suzuki, Hideo Fukano, Kazuo Shimozato, Shuji Hashimoto
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 46 Issue 5 Pg. 346-352 (May 2017) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 27605086 (Publication Type: Journal Article, Randomized Controlled Trial)
• Copyright: © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Biomarkers
• Ki-67 Antigen
• Tumor Suppressor Protein p53
• beta Carotene
• Ascorbic Acid

Topics

• Aged
• Ascorbic Acid (therapeutic use)
• Biomarkers (metabolism)
• Dietary Supplements
• Female
• Humans
• Ki-67 Antigen (metabolism)
• Leukoplakia, Oral (prevention & control)
• Male
• Treatment Outcome
• Tumor Suppressor Protein p53 (metabolism)
• beta Carotene (therapeutic use)