Abstract |
Huntington's disease (HD) is a hereditary neurodegenerative disease caused by a polyglutamine expansion within the huntingtin (HTT) gene. One of the cellular functions that is dysregulated in HD is store-operated calcium entry (SOCE), a process in which the depletion of Ca2+ from the endoplasmic reticulum (ER) induces Ca2+ influx from the extracellular space. We detected an enhanced activity of SOC channels in medium spiny neurons (MSNs) from YAC128 mice, a transgenic model of HD, and investigated whether this could be reverted by tetrahydrocarbazoles. The compound 6-bromo-N-(2-phenylethyl)-2,3,4,9-tetrahydro-1H-carbazol-1-amine hydrochloride was indeed able to restore the disturbed Ca2+ homeostasis and stabilize SOCE in YAC128 MSN cultures. We also detected a beneficial effect of this compound on the mitochondrial membrane potential. Since dysregulated Ca2+ homeostasis is believed to be one of the pathological hallmarks of HD, this compound might be a lead structure for HD treatment.
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Authors | Magdalena Czeredys, Filip Maciag, Axel Methner, Jacek Kuznicki |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 483
Issue 4
Pg. 1194-1205
(Feb 19 2017)
ISSN: 1090-2104 [Electronic] United States |
PMID | 27553284
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Carbazoles
- Culture Media
- Calcium
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Topics |
- Animals
- Calcium
(metabolism)
- Carbazoles
(pharmacology)
- Cells, Cultured
- Culture Media
- Endoplasmic Reticulum
(metabolism)
- Homeostasis
- Ion Transport
- Membrane Potential, Mitochondrial
(drug effects)
- Mice
- Mice, Transgenic
- Neurons
(drug effects, metabolism)
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