Tenuigenin exhibits protective effects against LPS-induced acute kidney injury via inhibiting TLR4/NF-κB signaling pathway.

Abstract

Tenuigenin (TNG) has been reported to have various pharmacological activities, such as anti-oxidative and anti-inflammatory activities. However, the protective effects of TNG on lipopolysaccharides (LPS)-induced acute kidney injury (AKI) are still not clear. The aim of this study was to investigate the protective effects and mechanism of TGN on LPS-induced AKI in mice. The kidney histological change, levels of blood urea nitrogen (BUN), and creatinine were measured to assess the protective effects of TNG on LPS-induced AKI. The levels of TNF-α, IL-1β, and IL-6 in serum and kidney tissues were detected by ELISA. The extent of nuclear factor kappa-B (NF-κB) p65 and the expression of Toll-like receptor-4 (TLR4) were detected by western blot analysis. The results showed that TNG markedly attenuated the histological alterations, BUN and creatinine levels in kidney. TNG also suppressed LPS-induced TNF-α, IL-1β, and IL-6 production. Furthermore, the expression of TLR4 and NF-κB activation induced by LPS were markedly inhibited by TNG. In conclusion, this study demonstrated that TNG protected against LPS-induced AKI by inhibiting TLR4/NF-κB signaling pathway.

• Authors: Haiyan Fu, Zhansheng Hu, Xingwei Di, Qiuhong Zhang, Rongbin Zhou, Hongyang Du
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 791 Pg. 229-234 (11 15 2016) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 27546562 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Cytokines
• Drugs, Chinese Herbal
• Lipopolysaccharides
• Toll-Like Receptor 4
• Transcription Factor RelA
• tenuigenin

Topics

• Acute Kidney Injury (chemically induced, drug therapy, pathology, physiopathology)
• Animals
• Cytokines (biosynthesis)
• Cytoprotection (drug effects)
• Drugs, Chinese Herbal (pharmacology)
• Gene Expression Regulation (drug effects)
• Kidney (drug effects, metabolism, pathology, physiopathology)
• Lipopolysaccharides (adverse effects)
• Male
• Mice
• Mice, Inbred BALB C
• Signal Transduction (drug effects)
• Toll-Like Receptor 4 (metabolism)
• Transcription Factor RelA (metabolism)