Abstract | BACKGROUND: METHODS: RESULTS: A significant time-group interaction was found for nociceptive thresholds (paw withdrawal threshold and paw withdrawal latency; all P < .0001). Remifentanil infusion induced distinct hyperalgesia at different time points (P < .0001), which was partly reversed by PICK1 knockdown (P < .007). Besides, remifentanil infusion increased the expression of PICK1 mRNA and protein (P < .0001) and the membrane GluR1 and GluR2 internalization in spinal dorsal horn neurons (P < .0011). More importantly, PICK1 deficiency could attenuate remifentanil-induced GluR2 internalization in the spinal cord dorsal horn (P < .01) but had no effect on remifentanil-induced membrane GluR1 expression (P ≥ .985). CONCLUSIONS: These results indicate that PICK1 deficiency might reverse remifentanil-induced hyperalgesia through regulating GluR2-containing AMPAR expression and trafficking in the spinal cord dorsal horn.
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Authors | Zhifen Wang, Yuan Yuan, Keliang Xie, Xiaohong Tang, Linlin Zhang, Jiying Ao, Nan Li, Yu Zhang, Suqian Guo, Guolin Wang |
Journal | Anesthesia and analgesia
(Anesth Analg)
Vol. 123
Issue 3
Pg. 771-81
(09 2016)
ISSN: 1526-7598 [Electronic] United States |
PMID | 27537764
(Publication Type: Journal Article)
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Chemical References |
- Analgesics, Opioid
- Carrier Proteins
- Cytoskeletal Proteins
- Nuclear Proteins
- PICK1 protein, rat
- Piperidines
- Receptors, AMPA
- Remifentanil
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Topics |
- Analgesics, Opioid
(toxicity)
- Animals
- Carrier Proteins
(physiology)
- Cytoskeletal Proteins
- Gene Expression Regulation
- Hyperalgesia
(chemically induced, metabolism)
- Male
- Nuclear Proteins
(physiology)
- Piperidines
(toxicity)
- Protein Transport
(drug effects, physiology)
- Rats
- Rats, Sprague-Dawley
- Receptors, AMPA
(biosynthesis, metabolism)
- Remifentanil
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