Abstract |
The down-regulation of microRNA-328a (miR-328a) in pentylenetetrazole (PTZ)-kindled rats with memory impairment was demonstrated in our previous study, while any contribution of miR-328a to cognitive dysfunction of PTZ-kindled rats remains unknown. In this study we have investigated the effect and the underlying mechanism of miR-328a on the cognitive function in PTZ-kindled rats. 48 SD male rats were divided into 4 groups as follows: a PTZ kindled group, a miR-328a antagomir group, an antagomir-control group, and a sham group (n=12 for each). All rats except those from the sham group were treated with PTZ 14 times at intervals of 48h to establish the temporal lobe epilepsy (TLE) models, and miR-328a antagomir was given to the antagomir group as a treatment by lateral-ventricle injection the day after the first injection of PTZ. Morris water maze (MWM) test was performed to assay their learning and memory abilities. The down-regulation of miR-328a in the PTZ group was confirmed using RT-qPCR and the expression of miR-328a was diminished after antagomir treatment (P<0.05). In the probe test of water maze, the time and distance of the PTZ group were both shorter than those of the sham group (P<0.05), and those of the antagomir-control group were both longer than those of the antagomir group (P<0.05). In addition, we found that with the down-regulation of miR-328a, the levels of Beta-site APP-cleaving enzyme (BACE), which is a bioinformatics-predicted target of miR-328a, were up-regulated. These findings suggest that miR-328a may play a role in memory dysfunction in PTZ-kindled rats by regulating the BACE levels and this links the PTZ model with Alzheimer's disease.
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Authors | Yuhan Liao, Yiqing Huang, Xixia Liu, Chun Luo, Donghua Zou, Xing Wei, Qi Huang, Yuan Wu |
Journal | Brain research bulletin
(Brain Res Bull)
Vol. 125
Pg. 205-10
(07 2016)
ISSN: 1873-2747 [Electronic] United States |
PMID | 27444122
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Antagomirs
- Bcl9l protein, rat
- GTPase-Activating Proteins
- MicroRNAs
- Neoplasm Proteins
- Syngap1 protein, rat
- Amyloid Precursor Protein Secretases
- Aspartic Acid Endopeptidases
- Bace1 protein, rat
- GTP-Binding Proteins
- Pentylenetetrazole
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Topics |
- Amyloid Precursor Protein Secretases
(metabolism)
- Animals
- Antagomirs
(pharmacology)
- Aspartic Acid Endopeptidases
(metabolism)
- Disease Models, Animal
- Down-Regulation
(drug effects)
- Epilepsy, Temporal Lobe
(chemically induced)
- GTP-Binding Proteins
(metabolism)
- GTPase-Activating Proteins
(metabolism)
- Kindling, Neurologic
(drug effects, physiology)
- Male
- Maze Learning
(drug effects, physiology)
- MicroRNAs
(chemistry, genetics, metabolism)
- Neoplasm Proteins
(metabolism)
- Pentylenetetrazole
(toxicity)
- Rats
- Rats, Sprague-Dawley
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