In recent years, the bioactive factors were utilized in exercise and athletic skin
injuries. In this research, the concentrated
conditioned medium of
hypoxia-preconditioned adipose mesenchymal stem cells, which is rich in bioactive factor, is applied in full-thickness skin defect model to evaluate the therapeutic efficacy. Adipose mesenchymal stem cells were harvested from the abdominal subcutaneous adipose tissues. The surface markers and the potential of differentiation were analyzed. The
conditioned medium of
hypoxia-preconditioned stem cells was collected and freeze-dried and then applied on the rat full-thickness skin defect model, and the healing time of each group was recorded. Haematoxylin and
eosin staining of skin was assessed by microscope. The characteristics of adipose mesenchymal stem cells were similar to those of other mesenchymal stem cells. The concentration of
protein in freeze-dried
conditioned medium in 1 mL water was about 15 times higher than in the normal condition medium. In vivo, the concentrated
hypoxia-preconditioned
conditioned medium can reduce the
wound size and accelerate the skin wound healing. The concentrated
hypoxia-preconditioned adipose mesenchymal stem cell-
conditioned medium has great effect on rat model of wound healing, and it would be an ideal agent for
wound care in clinical application.