Abstract |
Parkinson's disease (PD) is a neurodegenerative disorder, which can be modeled using the neurotoxin 6-hydroxydopamine (6-OHDA) to generate oxidative stress. Here, we studied the effects of the antioxidants deferricoprogen (DFC) and dimerumic acid (DMA), produced by rice fermented with Monascus purpureus NTU 568, on 6-OHDA-induced apoptosis in SH-SY5Y cells and their potential protective mechanisms. DMA and DFC inhibited 6-OHDA-induced apoptosis and cellular reactive oxygen species (ROS) in SH-SY5Y human neuroblastoma cells. Molecular analysis demonstrated associated upregulation of the Ak mouse strain thymoma (Akt), heme oxygenase-1 (HO-1), and signal-regulated kinase (ERK) pathways along with inhibited phosphorylation of c-Jun N-terminal kinase (JNK) and p38 pathways and altered homodimeric glycoprotein, N-methyl-d-aspartate ( NMDA) receptor, and immunoglobulin Fc receptor gene expression. These results suggested that the neuroprotection elicited by DMA and DFC against 6-OHDA-induced neurotoxicity was associated with the Akt, MAPK, and HO-1 pathways via regulating the gene expression of NMDA receptor, homodimeric glycoprotein, and immunoglobulin Fc receptor.
|
Authors | Wei-Ting Tseng, Ya-Wen Hsu, Tzu-Ming Pan |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 64
Issue 30
Pg. 5995-6002
(Aug 03 2016)
ISSN: 1520-5118 [Electronic] United States |
PMID | 27431098
(Publication Type: Journal Article)
|
Chemical References |
- Antioxidants
- Diketopiperazines
- Hydroxamic Acids
- Reactive Oxygen Species
- deferricoprogen
- dimerumic acid
- Oxidopamine
- Heme Oxygenase-1
- Proto-Oncogene Proteins c-akt
|
Topics |
- Antioxidants
(therapeutic use)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Cell Survival
- Diketopiperazines
(chemistry)
- Heme Oxygenase-1
(metabolism)
- Humans
- Hydroxamic Acids
(chemistry)
- Oryza
(chemistry)
- Oxidative Stress
- Oxidopamine
(chemistry)
- Parkinson Disease
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Reactive Oxygen Species
(metabolism)
|