Evaluation of potential genotoxic/cytotoxic effects induced by epoxiconazole and fenpropimorph-based fungicide in bovine lymphocytes in vitro.

Abstract

Potential genotoxic/cytotoxic effects of the epoxiconazole/fenpropimorph-based fungicide were investigated using single cell gel electrophoresis and cytogenetic assays: chromosomal aberrations, sister chromatid exchanges, micronuclei and fluorescence in situ hybridization in cultured bovine lymphocytes. No statistically significant elevations of DNA damage and increases in cytogenetic endpoints were seen. However, evident cytotoxic effect presented as a decrease in mitotic and proliferation indices were recorded after exposure of bovine lymphocytes to the fungicide for 24 and 48 h at concentrations ranging from 3 to 15 µg mL(-1) (P < 0.05, P < 0.01, P < 0.001). Similarly, for 24 h an inhibition in the cytokinesis block proliferation index (CBPI) was obtained after exposure to the fungicide at concentrations ranging from 1.5 to 15 µg mL(-1) (P < 0.01, P < 0.001) in each donor.

• Authors: Monika Drážovská, Katarína Šiviková, Beáta Holečková, Ján Dianovský, Martina Galdíková, Viera Schwarzbacherová
• Journal: Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes (J Environ Sci Health B) Vol. 51 Issue 11 Pg. 769-76 (Nov 2016) ISSN: 1532-4109 [Electronic] England
• PMID: 27428828 (Publication Type: Journal Article)

Chemical References

• Cytotoxins
• Epoxy Compounds
• Fungicides, Industrial
• Morpholines
• Mutagens
• Triazoles

Topics

• Animals
• Cattle
• Cells, Cultured (drug effects)
• Chromosome Aberrations (drug effects)
• Cytotoxins (toxicity)
• DNA Damage (drug effects)
• Epoxy Compounds (toxicity)
• Fungicides, Industrial (pharmacology, toxicity)
• Humans
• In Situ Hybridization, Fluorescence
• Lymphocytes (drug effects)
• Mitotic Index
• Morpholines (toxicity)
• Mutagenicity Tests
• Mutagens (toxicity)
• Sister Chromatid Exchange (drug effects)
• Triazoles (toxicity)