Evaluation of Oxidative Stress Parameters and Energy Metabolism in Cerebral Cortex of Rats Subjected to Sarcosine Administration.

Abstract

Sarcosine is an N-methyl derivative of the amino acid glycine, and its elevation in tissues and physiological fluids of patients with sarcosinemia could reflect a deficient pool size of activated 1-carbon units. Sarcosinemia is a rare inherited metabolic condition associated with mental retardation. In the present study, we investigated the acute effect of sarcosine and/or creatine plus pyruvate on some parameters of oxidative stress and energy metabolism in cerebral cortex homogenates of 21-day-old Wistar rats. Acute administration of sarcosine induced oxidative stress and diminished the activities of adenylate kinase, GAPDH, complex IV, and mitochondrial and cytosolic creatine kinase. On the other hand, succinate dehydrogenase activity was enhanced in cerebral cortex of rats. Moreover, total sulfhydryl content was significantly diminished, while DCFH oxidation, TBARS content, and activities of SOD and GPx were significantly enhanced by acute administration of sarcosine. Co-administration of creatine plus pyruvate was effective in the prevention of alterations provoked by sarcosine administration on the oxidative stress and the enzymes of phosphoryltransfer network. These results indicate that acute administration of sarcosine may stimulate oxidative stress and alter the energy metabolism in cerebral cortex of rats. In case these effects also occur in humans, they may contribute, along with other mechanisms, to the neurological dysfunction of sarcosinemia, and creatine and pyruvate supplementation could be beneficial to the patients.

• Authors: Rodrigo Binkowski de Andrade, Tanise Gemelli, Denise Bertin Rojas, Tomas Duk Hwa Kim, Ângela Zanatta, Felipe Schmitz, André Felipe Rodrigues, Angela T S Wyse, Moacir Wajner, Carlos Severo Dutra-Filho, Clovis Milton Duval Wannmacher
• Journal: Molecular neurobiology (Mol Neurobiol) Vol. 54 Issue 6 Pg. 4496-4506 (08 2017) ISSN: 1559-1182 [Electronic] United States
• PMID: 27356917 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluoresceins
• 2',7'-dichlorodihydrofluorescein
• Glutathione Peroxidase
• Superoxide Dismutase
• Creatine Kinase
• Adenylate Kinase
• Sarcosine

Topics

• Adenylate Kinase (metabolism)
• Animals
• Cerebral Cortex (metabolism, pathology)
• Creatine Kinase (metabolism)
• Energy Metabolism
• Fluoresceins (metabolism)
• Glutathione Peroxidase (metabolism)
• Models, Biological
• Oxidation-Reduction
• Oxidative Stress
• Rats, Wistar
• Sarcosine (administration & dosage)
• Superoxide Dismutase (metabolism)