Abstract | BACKGROUND: RESULTS: We show that the disease causing mutations in patient-derived fibroblasts do not affect subcellular localization of TRNT1 and show no gross morphological differences when compared to control cells. Analysis of cellular respiration and oxidative phosphorylation (OXPHOS) complexes demonstrates that both basal and maximal respiration rates are decreased in patient cells, which may be attributed to an observed decrease in the abundance of select proteins of the OXPHOS complexes. CONCLUSIONS: Our data provides further insight into cellular pathophysiology of SIFD.
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Authors | Urszula Liwak-Muir, Hapsatou Mamady, Turaya Naas, Quinlan Wylie, Skye McBride, Matthew Lines, Jean Michaud, Stephen D Baird, Pranesh K Chakraborty, Martin Holcik |
Journal | Orphanet journal of rare diseases
(Orphanet J Rare Dis)
Vol. 11
Issue 1
Pg. 79
(06 18 2016)
ISSN: 1750-1172 [Electronic] England |
PMID | 27317422
(Publication Type: Journal Article)
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Chemical References |
- Nucleotidyltransferases
- TRNT1 protein, human
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Topics |
- Anemia, Sideroblastic
(genetics, metabolism)
- Blotting, Western
- Cell Respiration
(genetics, physiology)
- Cells, Cultured
- Female
- Fibroblasts
(metabolism)
- Fluorescent Antibody Technique
- Humans
- Membrane Potential, Mitochondrial
- Microscopy, Electron, Transmission
- Mitochondria
(metabolism)
- Mutation
- Nucleotidyltransferases
(genetics, metabolism)
- Oxidative Phosphorylation
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