Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways.

Abstract

In the current study, we analyzed the functions and mechanisms of Bletilla striata polysaccharide b (BSPb) against Angiotensin II (Ang II)-induced oxidative stress and inflammation in human mesangial cells (HMCs). It was found that BSPb could inhibit generation of Ang II-induced reactive oxygen species (ROS) and activation of proinflammatory cytokines interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) in a dose-dependent manner. Further studies revealed that BSPb effectively blocked upregulation of NADPH oxidase 4 (NOX4). Moreover, knockdown of NOX4 significantly impaired the anti-oxidative function of BSPb. In addition, BSPb decreased overexpression of Toll-like receptor 2 (TLR2) induced by Ang II. Blocking TLR2 expression impaired the anti-inflammatory effects of BSPb. In conclusion, BSPb was found to possess anti-oxidative stress and anti-inflammatory functions against Ang II-induced ROS generation and proinflammatory cytokines activation. The NOX4 and TLR2 pathways played important roles in the biological effects mediated by BSPb.

• Authors: Long Yue, Wang Wang, Yan Wang, Ting Du, Weiping Shen, Huiling Tang, Ying Wang, Hongping Yin
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 89 Pg. 376-88 (Aug 2016) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 27151672 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Plant Extracts
• Polysaccharides
• Reactive Oxygen Species
• TLR2 protein, human
• Toll-Like Receptor 2
• Angiotensin II
• NADPH Oxidase 4
• NADPH Oxidases
• NOX4 protein, human

Topics

• Angiotensin II (drug effects, metabolism)
• Cell Line
• Gene Expression Regulation (drug effects)
• Humans
• Inflammation (drug therapy, genetics, pathology)
• Mesangial Cells (drug effects, metabolism)
• NADPH Oxidase 4
• NADPH Oxidases (biosynthesis, genetics)
• Orchidaceae (chemistry)
• Oxidative Stress (drug effects)
• Plant Extracts (administration & dosage, chemistry)
• Polysaccharides (administration & dosage, chemistry)
• Reactive Oxygen Species (metabolism)
• Signal Transduction (drug effects)
• Toll-Like Receptor 2 (biosynthesis, genetics)