Perioperative time course of matrix metalloproteinase-9 (MMP-9), its tissue inhibitor TIMP-1 & S100B protein in carotid surgery.

Abstract

BACKGROUND & OBJECTIVES:

Ischaemic stroke is a life burdening disease for which carotid endarterectomy (CEA) is considered a gold standard intervention. Pro-inflammatory markers like matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) and S-100 Beta (S100B) may have a role in the early inflammation and cognitive decline following CEA. This study was aimed to describe the perioperative time courses and correlations between of MMP-9, TIMP-1 and S100B following CEA.

METHODS:

Fifty four patients scheduled for CEA were enrolled. Blood samples were collected at four time points, T 1 : preoperative, T 2 : 60 min after cross-clamp release, T 3 : first postoperative morning, T 4 : third postoperative morning. Twenty atherosclerotic patients were included as controls. Plasma MMP-9, TIMP-1 and S100B levels were estimated by ELISA.

RESULTS:

TIMP-1 was decreased significantly in the CEA group (P<0.01). Plasma MMP-9 was elevated and remained elevated from T 1-4 in the CEA group (P<0.05) with a marked elevation in T 3 compared to T 1 (P<0.05). MMP-9/TIMP-1 was elevated in the CEA group and increased further by T 2 and T 3 (P<0.05). S100B was elevated on T 2 and decreased on T 3-4 compared to T 1 .

INTERPRETATION & CONCLUSIONS:

Our study provides information on the dynamic changes of MMP-9-TIMP-1 system and S100B in the perioperative period. Preoperative reduction of TIMP-1 might be predictive for shunt requirement but future studies are required for verification.

• Authors: Bálint Nagy, Gábor Woth, Ákos Mérei, Lilla Nagy, János Lantos, Gábor Menyhei, Lajos Bogár, Diána Mühl
• Journal: The Indian journal of medical research (Indian J Med Res) Vol. 143 Issue 2 Pg. 220-6 (Feb 2016) ISSN: 0971-5916 [Print] India
• PMID: 27121520 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• S100 Calcium Binding Protein beta Subunit
• S100B protein, human
• TIMP1 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Aged
• Biomarkers (blood)
• Carotid Arteries (physiopathology, surgery)
• Endarterectomy, Carotid (adverse effects)
• Female
• Humans
• Inflammation (blood, physiopathology)
• Male
• Matrix Metalloproteinase 9 (blood)
• Middle Aged
• Perioperative Period
• S100 Calcium Binding Protein beta Subunit (blood)
• Stroke (blood, physiopathology, surgery)
• Tissue Inhibitor of Metalloproteinase-1 (blood)