Estrogen induces multiple regulatory B cell subtypes and promotes M2 microglia and neuroprotection during experimental autoimmune encephalomyelitis.

Abstract

Sex hormones promote immunoregulatory effects on multiple sclerosis. The current study evaluated estrogen effects on regulatory B cells and resident CNS microglia during experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate an estrogen-dependent induction of multiple regulatory B cell markers indicative of IL-10 dependent as well as IFN-γ dependent pathways. Moreover, although estrogen pretreatment of EAE mice inhibited the infiltration of pro-inflammatory cells into the CNS, it enhanced the frequency of regulatory B cells and M2 microglia. Our study suggests that estrogen has a broad effect on the development of regulatory B cells during EAE, which in turn could promote neuroprotection.

• Authors: Gil Benedek, Jun Zhang, Sheetal Bodhankar, Ha Nguyen, Gail Kent, Kelley Jordan, Dustin Manning, Arthur A Vandenbark, Halina Offner
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 293 Pg. 45-53 (Apr 15 2016) ISSN: 1872-8421 [Electronic] Netherlands
• PMID: 27049561 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Cytokines
• Myelin-Oligodendrocyte Glycoprotein
• Peptide Fragments
• RNA, Messenger
• myelin oligodendrocyte glycoprotein (35-55)
• Estradiol
• Freund's Adjuvant
• Pertussis Toxin

Topics

• Animals
• B-Lymphocytes, Regulatory (drug effects, metabolism)
• Brain (pathology)
• Cell Proliferation (drug effects)
• Cytokines (genetics, metabolism)
• Disease Models, Animal
• Encephalomyelitis, Autoimmune, Experimental (chemically induced, drug therapy, pathology)
• Estradiol (therapeutic use)
• Freund's Adjuvant (toxicity)
• Leukocytes (drug effects, metabolism)
• Mice
• Mice, Inbred C57BL
• Microglia (drug effects, metabolism)
• Myelin-Oligodendrocyte Glycoprotein (immunology, toxicity)
• Peptide Fragments (immunology, toxicity)
• Pertussis Toxin (toxicity)
• RNA, Messenger (metabolism)
• Spinal Cord (cytology)
• Spleen (cytology)
• Statistics, Nonparametric
• Time Factors