Investigation of N-acetylcysteine on contralateral testis tissue injury by experimental testicular torsion: long-term effect.

Abstract

BACKGROUND:

The aim of the study was to investigate long-term effects of N-acetylcysteine (NAC) on contralateral testes by experimental testicular torsion using histopathologic and biochemical parameters.

METHODS:

Eighteen rats were randomized and divided into 3 groups. In group 1, the control group (C), laparotomy was performed and the left and right testes were excised 2 months later. In group 2, the torsion and detorsion group (T), the torsion was performed by rotating the left testis 720° to clockwise direction, and then 4 hours later, detorsion was performed; 2 months later, contralateral testes were removed. In group 3, the NAC adding torsion and detorsion group (T+NAC), the torsion was performed by rotating the left testis 720° to clockwise direction, and then 4 hours later, detorsion was performed. N-acetylcysteine was given intraperitoneally 30 minutes before detorsion and following 5 days after detorsion.

RESULTS:

GPx activities were increased in the T and T+NAC groups compared with the control (P = .008 and P = .016, respectively). Seminiferous tubule diameter thickness is decreased in the torsion group compared with the control group and decreased in the T+NAC group compared with the torsion group (P < .05).

CONCLUSION:

In the long term as implied from the histopathologic findings, NAC has beneficial effects against contralateral testis tissue injury induced by testicular torsion.

• Authors: Akın Bodur, Ahmet Alver, Cemil Kahraman, Diler Us Altay, İmran İnce
• Journal: The American journal of emergency medicine (Am J Emerg Med) Vol. 34 Issue 6 Pg. 1069-74 (Jun 2016) ISSN: 1532-8171 [Electronic] United States
• PMID: 27037130 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Free Radical Scavengers
• Malondialdehyde
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase
• Glutathione
• Acetylcysteine

Topics

• Acetylcysteine (therapeutic use)
• Animals
• Catalase (metabolism)
• Disease Models, Animal
• Free Radical Scavengers (therapeutic use)
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• Male
• Malondialdehyde (metabolism)
• Rats
• Spermatic Cord Torsion (complications, metabolism, pathology)
• Superoxide Dismutase (metabolism)
• Testicular Diseases (etiology, prevention & control)
• Time Factors