Aggravation of brain infarction through an increase in acrolein production and a decrease in glutathione with aging.

Abstract

We previously reported that tissue damage during brain infarction was mainly caused by inactivation of proteins by acrolein. This time, it was tested why brain infarction increases in parallel with aging. A mouse model of photochemically induced thrombosis (PIT) was studied using 2, 6, and 12 month-old female C57BL/6 mice. The size of brain infarction in the mouse PIT model increased with aging. The volume of brain infarction in 12 month-old mice was approximately 2-fold larger than that in 2 month-old mice. The larger brain infarction in 12 month-old mice was due to an increase in acrolein based on an increase in the activity of spermine oxidase, together with a decrease in glutathione (GSH), a major acrolein-detoxifying compound in cells, based on the decrease in one of the subunits of glutathione biosynthesizing enzymes, γ-glutamylcysteine ligase modifier subunit, with aging. The results indicate that aggravation of brain infarction with aging was mainly due to the increase in acrolein production and the decrease in GSH in brain.

• Authors: Takeshi Uemura, Kenta Watanabe, Misaki Ishibashi, Ryotaro Saiki, Kyoshiro Kuni, Kazuhiro Nishimura, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 473 Issue 2 Pg. 630-5 (Apr 29 2016) ISSN: 1090-2104 [Electronic] United States
• PMID: 27037020 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Acrolein
• Oxidoreductases Acting on CH-NH Group Donors
• polyamine oxidase
• Glutathione

Topics

• Acrolein (metabolism)
• Aging
• Animals
• Brain (metabolism, pathology)
• Brain Infarction (metabolism, pathology)
• Female
• Glutathione (metabolism)
• Mice
• Mice, Inbred C57BL
• Oxidoreductases Acting on CH-NH Group Donors (metabolism)