Autotetraploid cell line induced by SP600125 from crucian carp and its developmental potentiality.

Abstract

Polyploidy has many advantages over diploidy, such as rapid growth, sterility, and disease resistance, and has been extensively applied in agriculture and aquaculture. Though generation of new polyploids via polyploidization has been achieved in plants by different ways, it is comparatively rare in animals. In this article, by a chemical compound, SP600125, polyploidization is induced in fish cells in vitro, and a stable autotetraploid cell line has been generated from diploid fibroblast cells of crucian carp. As a c-Jun N-terminal kinase (Jnk) inhibitor, SP600125 does not function during the induction process of polyploidization. Instead, the p53 signal pathway might be involved. Using the SP600125-induced tetraploid cells and eggs of crucian carp as the donors and recipients, respectively, nuclear transplantation was conducted such that tetraploid embryos were obtained. It suggests that combining polyploidization and the somatic cell nuclear transfer technique (SCNT) is an efficient way to generate polyploidy, and the presented method in this research for generating the tetraploid fish from diploid fish can provide a useful platform for polyploid breeding.

• Authors: Yonghua Zhou, Mei Wang, Minggui Jiang, Liangyue Peng, Cong Wan, Jinhui Liu, Wenbin Liu, Rurong Zhao, Xiaoyang Zhao, Wei Hu, Shaojun Liu, Yamei Xiao
• Journal: Scientific reports (Sci Rep) Vol. 6 Pg. 21814 (Feb 22 2016) ISSN: 2045-2322 [Electronic] England
• PMID: 26898354 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthracenes
• Fish Proteins
• Mutagens
• Tumor Suppressor Protein p53
• pyrazolanthrone
• JNK Mitogen-Activated Protein Kinases

Topics

• Animals
• Animals, Genetically Modified
• Anthracenes (pharmacology)
• Aquaculture (methods)
• Carps (genetics)
• Cell Line
• Diploidy
• Embryo, Nonmammalian
• Female
• Fish Proteins (antagonists & inhibitors, genetics, metabolism)
• Founder Effect
• Gene Expression Regulation
• JNK Mitogen-Activated Protein Kinases (antagonists & inhibitors, genetics, metabolism)
• Karyotyping
• Male
• Mutagens (pharmacology)
• Nuclear Transfer Techniques
• Tetraploidy
• Tumor Suppressor Protein p53 (genetics, metabolism)
• Zygote (growth & development, metabolism)