Abstract | PURPOSE: The purpose of this study was to compare the cytotoxicity and antiinflammatory effect of preserved and unpreserved 0.1% fluorometholone ( FML). METHODS: RESULTS: BAC and preserved FML caused cell shrinkage and detachment from the plate in a dose-dependent manner, and cell viability decreased significantly. However, cytotoxicity was reduced on treatment with unpreserved FML. Hematoxylin- eosin staining revealed surface desquamation, irregular surface, loss of cell borders, and stromal shrinkage in the group treated with BAC. On BAC exposure, tumor necrosis factor-α, interleukin-6, and human leukocyte antigen-DR were strongly detected, and cytotoxicity was markedly increased, as evidenced by a positive result in the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Ocular surface damage and inflammation were slightly reduced on treatment with preserved FML. In comparison, unpreserved FML did not induce morphological changes; moreover, decreased cell cytotoxicity and ocular surface inflammation were observed. CONCLUSIONS: The cytotoxicity of antiinflammatory eye drops evaluated in this study was induced by the preservative BAC. Accordingly, unpreserved FML is more effective than preserved eye drops in decreasing ocular inflammation.
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Authors | Yeoun-Hee Kim, Jae-Chang Jung, Soon-Young Jung, Sung Yu, Kyoo Won Lee, Young Jeung Park |
Journal | Cornea
(Cornea)
Vol. 35
Issue 2
Pg. 234-42
(Feb 2016)
ISSN: 1536-4798 [Electronic] United States |
PMID | 26619385
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzalkonium Compounds
- Glucocorticoids
- HLA-DR Antigens
- Interleukin-6
- Ophthalmic Solutions
- Preservatives, Pharmaceutical
- Tumor Necrosis Factor-alpha
- Fluorometholone
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Topics |
- Administration, Topical
- Animals
- Benzalkonium Compounds
(toxicity)
- Cell Line
- Cell Survival
- Dose-Response Relationship, Drug
- Dry Eye Syndromes
(drug therapy, metabolism)
- Epithelium, Corneal
(drug effects, metabolism)
- Female
- Fluorescent Antibody Technique, Indirect
- Fluorometholone
(toxicity)
- Glucocorticoids
(toxicity)
- HLA-DR Antigens
(metabolism)
- In Situ Nick-End Labeling
- Interleukin-6
(metabolism)
- Mice
- Mice, Inbred C57BL
- Ophthalmic Solutions
- Preservatives, Pharmaceutical
(toxicity)
- Treatment Outcome
- Tumor Necrosis Factor-alpha
(metabolism)
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