Essential Oil Composition, Antioxidant, Cytotoxic and Antiviral Activities of Teucrium pseudochamaepitys Growing Spontaneously in Tunisia.

Abstract

The chemical composition, antioxidant, cytotoxic and antiviral activities of the essential oil obtained by hydrodistillation from the aerial parts of Teucrium pseudochamaepitys (Lamiaceae) collected from Zaghouan province of Tunisia are reported. The essential oil was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC/MS). Thirty-one compounds were identified representing 88.6% of the total essential oil. Hexadecanoic acid was found to be the most abundant component (26.1%) followed by caryophyllene oxide (6.3%), myristicin (4.9%) and α-cubebene (3.9%). The antioxidant capacity of the oil was measured on the basis of the scavenging activity to the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH). The IC50 value of the oil was evaluated as 0.77 mg·mL(-1). In addition, the essential oil was found to possess moderate cytotoxic effects on the HEp-2 cell line (50% cytotoxic concentration (CC50)=653.6 µg·mL(-1)). The potential antiviral effect was tested against Coxsackievirus B (CV-B), a significant human and mouse pathogen that causes pediatric central nervous system disease, commonly with acute syndromes. The reduction of viral infectivity by the essential oil was measured using a cytopathic (CPE) reduction assay.

• Authors: Saoussen Hammami, Habib Jmii, Ridha El Mokni, Abdelbaki Khmiri, Khaled Faidi, Hatem Dhaouadi, Mohamed Hédi El Aouni, Mahjoub Aouni, Rajesh K Joshi
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 20 Issue 11 Pg. 20426-33 (Nov 16 2015) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 26580590 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Antiviral Agents
• Free Radical Scavengers
• Oils, Volatile
• Plant Extracts

Topics

• Antioxidants (chemistry, pharmacology)
• Antiviral Agents (chemistry, pharmacology)
• Cell Line
• Cell Survival (drug effects)
• Enterovirus B, Human (drug effects)
• Free Radical Scavengers (chemistry, pharmacology)
• Humans
• Inhibitory Concentration 50
• Microbial Sensitivity Tests
• Oils, Volatile (chemistry, pharmacology)
• Plant Extracts (chemistry, pharmacology)
• Teucrium (chemistry)
• Tunisia
• Viral Plaque Assay