Homing of allogeneic nestin-positive hair follicle-associated pluripotent stem cells after maternal transplantation in experimental model of cortical dysplasia.

Abstract

An embryo has the capability to accept allo- or xeno-geneic cells, which probably makes it an ideal candidate for stem cell transplantation of various cerebral cortex abnormalities, such as cortical dysplasia. The aim of this study was to determine hair follicle-associated pluripotent (HAP) stem cells homing into various organs of mother and fetus. Cells were obtained, analyzed for immunophenotypic features, and then labelled with CM-Dil; nestin(+)HAP stem cells or media phosphate-buffered saline (PBS) were intravenously delivered on day 16 of gestation in BALB/c mice, which intraperitoneally received methylazoxymethanol (MAM) one day in advance, and homing was assessed at 24 h after cell injection. Flow cytometry and immunocytochemistry manifested positive expression of nestin in HAP stem cells. For both mother and fetus, brain, lungs, liver, and spleen were the host organs for cell implants. For the brain, the figure was considerably higher in fetus, 4.05 ± 0.5% (p ≤ 0.05 vs. mother). MAM-injected mice had a downward trend for SDF-1α and CXCR4 (p ≤ 0.05 vs. control), but HAP stem cells group showed an upward trend for CXCR4 (p ≤ 0.05 vs. MAM). We conclude the HAP stem cells show homing potential in experimental cortical dysplasia, which may permit these cells to be a target in future work on prenatal therapy of neural disorders.

• Authors: Ameneh Omidi, Iraj Ragerdi Kashani, Mohammad Akbari, Keywan Mortezaee, Soudabeh Ghasemi, Cordian Beyer, Adib Zendedel
• Journal: Biochemistry and cell biology = Biochimie et biologie cellulaire (Biochem Cell Biol) Vol. 93 Issue 6 Pg. 619-25 (Dec 2015) ISSN: 1208-6002 [Electronic] Canada
• PMID: 26568364 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3,3'-dioactadecyl-5,5'-di(4-sulfophenyl)oxacarbocyanine
• Carbocyanines
• Fluorescent Dyes
• Nes protein, mouse
• Nestin

Topics

• Animals
• Brain (embryology, immunology, metabolism, pathology)
• Carbocyanines (chemistry)
• Disease Models, Animal
• Female
• Fetal Therapies
• Flow Cytometry
• Fluorescent Dyes (chemistry)
• Hair Follicle (cytology)
• Immunohistochemistry
• Malformations of Cortical Development (embryology, immunology, pathology, therapy)
• Maternal-Fetal Exchange
• Mice, Inbred BALB C
• Microscopy, Fluorescence
• Nestin (metabolism)
• Neurons (immunology, metabolism, pathology)
• Pluripotent Stem Cells (cytology, immunology, metabolism, transplantation)
• Pregnancy
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction