Licochalcone A Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting NF-κB Activation.

Abstract

Licochalcone A (Lico A), a flavonoid found in licorice root (Glycyrrhiza glabra), has been reported to have anti-inflammatory activity. However, the protective effects of Lico A on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) remains unclear. In this study, using a mouse model of LPS-induced AKI, we investigated the protective effects and mechanism of Lico A on LPS-induced AKI in mice. LPS-induced kidney injury was assessed by detecting kidney histological study, blood urea nitrogen (BUN), and creatinine levels. The production of inflammatory cytokines TNF-α, IL-6, and IL-1β in serum and kidney tissues was detected by ELISA. The activation of NF-κB was measured by western blot analysis. Our results showed that Lico A dose-dependently attenuated LPS-induced kidney histopathologic changes, serum BUN, and creatinine levels. Lico A also suppressed LPS-induced TNF-α, IL-6, and IL-1β production both in serum and kidney tissues. Furthermore, our results showed that Lico A significantly inhibited LPS-induced NF-κB activation. In conclusion, our results suggest that Lico A has protective effects against LPS-induced AKI and Lico A exhibits its anti-inflammatory effects through inhibiting LPS-induced NF-κB activation.

• Authors: Jun Hu, Jun Liu
• Journal: Inflammation (Inflammation) Vol. 39 Issue 2 Pg. 569-74 (Apr 2016) ISSN: 1573-2576 [Electronic] United States
• PMID: 26552405 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Chalcones
• IL1B protein, mouse
• Interleukin-1beta
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Plant Preparations
• Tumor Necrosis Factor-alpha
• Creatinine
• licochalcone A

Topics

• Acute Kidney Injury (chemically induced, drug therapy)
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Blood Urea Nitrogen
• Chalcones (therapeutic use)
• Creatinine (blood)
• Disease Models, Animal
• Enzyme Activation (drug effects)
• Fabaceae (metabolism)
• Female
• Interleukin-1beta (biosynthesis, blood)
• Interleukin-6 (biosynthesis, blood)
• Kidney (pathology)
• Lipopolysaccharides
• Mice
• Mice, Inbred C57BL
• NF-kappa B (metabolism)
• Plant Preparations (therapeutic use)
• Tumor Necrosis Factor-alpha (biosynthesis, blood)