Using a rat
penetrating keratoplasty model, this study aims to explore the inhibitory effect of
hachimycin on corneal graft rejection, to provide new basis for its clinical application.
MATERIALS AND METHODS: RESULTS:
Penetrating keratoplasty was successfully performed on all the 3 groups of rats. Five days after the
keratoplasty, both the transparency and the implant
edema showed a score of 1-2 degrees in group A and B. Two weeks later, both these two grafts components increased to a score of 2-3 degrees in group A and B, with an active neovascularization. The group C also showed a transparency and implant
edema of 1-2 degrees five days after the
keratoplasty surgery. However, a transparent implant without
edema was observed in group C two weeks after the
keratoplasty surgery. In addition, the newly formed blood vessels disappeared and the retina appeared in a good status and in the correct position. The corneal transparency,
edema, corneal neovascularization, and total RI scores of the 3 groups clearly indicated that the group B showed an improvement compared to the group A (P < 0.05), since in group B the new vessels were only distributed in the corneal limbus at five days after the surgery. However, at two weeks after the surgery no statistically significant difference in neovascularization degree was observed in group B when compared with group A, while a statistically significant decrease of neovascularization was observed in group C (P < 0.05).
CONCLUSIONS: