Antiproliferative Constituents of Geopropolis from the Bee Melipona scutellaris.

Abstract

Fractionation of geopropolis from Melipona scutellaris, guided by antiproliferative activity against two colon cancer cell lines (COLO205 and KM12), led to the isolation of two new cinnamic acid esters, mammea-type coumarins 5,7-dihydroxy-6-(3-methyl-2-butenyl)-8-(4-cinnamoyl-3-methyl-1-oxobutyl)-4-propyl-coumarin (1) and 5,7-dihydroxy-6-(4-cinnamoyl-3-methyl-1-oxobutyl)-4-phenylcoumarin (2), along with five known coumarins, mammeigin (3), hydroxymammeigin (4), mammeisin (5), cinnamoyloxy-mammeisin (6), and mammein (7), and the prenylated benzophenone ent-nemorosone (8). Among the isolated compounds, 5 and 7 showed the highest cell growth inhibition against COLO205 (GI50 9.7 and 10.7 µM, respectively) and KM12 (GI50 12.0 and 10.9 µM, respectively). The presence of these compounds suggests that plants of Clusiaceae family, especially the genera Kielmeyera and Clusia, are likely to be major sources of geopropolis produced by M. scutellaris.

• Authors: Marcos Guilherme da Cunha, Pedro Luiz Rosalen, Marcelo Franchin, Severino Matias de Alencar, Masaharu Ikegaki, Tanya Ransom, John Albert Beutler
• Journal: Planta medica (Planta Med) Vol. 82 Issue 3 Pg. 190-4 (Feb 2016) ISSN: 1439-0221 [Electronic] Germany
• PMID: 26544117 (Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Antineoplastic Agents
• Coumarins
• Propolis

Topics

• Animals
• Antineoplastic Agents (isolation & purification, pharmacology)
• Bees (chemistry)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Coumarins (isolation & purification, pharmacology)
• Drug Screening Assays, Antitumor
• Humans
• Propolis (chemistry)