Cardiomyocyte
hypertrophy is a threat to human health due to the probability of sudden heart failure‑induced mortality. Previous studies have demonstrated that nuclear factor of activated T cells cytoplasmic 3 (
NFATC3) is important in the process of cardiomyocyte
hypertrophy. However, the molecular mechanism underlying the alteration in the expression levels of
NFATC3 during cardiomyocyte
hypertrophy has remained to be fully elucidated. In order to shed light on the molecular mechanism, the present study employed several approaches, including the measurement of the cell surface area, analysis of the
protein/
DNA ratio, western blot analysis and a
Luciferase reporter assay using isolated rat cardiomyocytes as model. The results showed that expression of microRNA‑1 (miR‑1) was reduced in patients diagnosed with
cardiac hypertrophy and rat cardiomyocytes treated with pro‑hypertrophic stimuli. The increase in the expression of miR‑1 was able to inhibit the hypertrophic remodeling of cardiomyocytes. The suppression of miR‑1 was sufficient to induce cardiomyocyte
hypertrophy, and further experiments confirmed that
NFATC3 was a target of miR‑1 in cardiomyocytes. Forced expression of
NFATC3 inhibited the protective activity of miR‑1 against hypertrophic stimuli in the cardiomyocytes. These findings provided clarification of the regulatory signaling pathway underlying
cardiac hypertrophy, and provided evidence that targeting the miR‑1/
NFATC3 pathway may be a promising strategy for the prevention and treatment of
heart hypertrophy.