Bis-
nitroxide malonate methanofullerene (NO)2-MF was studied as a biomimetic model of reduction-oxidation activity with natural compounds-
cytochrome c (cyt c),
dihydroquercetin (DHQ),
ascorbic acid (AA) and synthetic drug-1-(β-oxyethyl)-4,6-dimethyl-1,2-dihydro-2-oxopyrimidine (
xymedon(®)). (NO)2-MF may be used as the component of Langmuir monolayers on an aqueous subphase and as the adsorbate on
silica gel. The activity of (NO)2-MF in the reaction with cyt c was compared with the effect of
nitroxide species such as gaseous
nitric oxide,
2,2,6,6-tetramethylpiperidine-1-oxyl radical (
TEMPO) by using UV-vis and EPR-spectra. It has been shown, that
iron(III) in cyt c(3+) under action (NO)2-MF was reduced up to
iron(II), similar effect was observed under the influence of gaseous NO in aqueous
solution, but reduction of
iron(III) in
heme cyt c was reversible in the presence of
TEMPO. Therefore, the state of Fe-
heme in cyt c can be used as the
indicator of the interaction of cyt c with
nitroxide species in vitro. The interaction of cyt c, DHQ,
xymedon(®) with (NO)2-MF monolayers was confirmed by the increasing of limiting area А0 from 0.88 nm(2) up to 1.70 nm(2) of (NO)2-MF on the aqueous subphase, by the paramagnetism and UV-vis spectral data changes. These results can be explained by appearance of oxoammonium ion (NO(+))2-MF adlayers and monolayers. The
antioxidant and regenerating effects were shown when treating
wounds by
xymedon(®) in the presence of additives (0.001%) of (NO)2-MF in the experiments on the rats.