Duffy blood group genotyping in Thai blood donors.

Abstract

BACKGROUND:

Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible blood for alloimmunized patients was assessed.

METHODS:

Five hundred blood samples from Thai blood donors of the National Blood Centre, Thai Red Cross Society, were included. Only 200 samples were tested with anti-Fy(a) and anti-Fy(b) using the gel technique. All 500 samples and four samples from a Guinea family with the Fy(a-b-) phenotype were genotyped by using PCR-SSP. Additionally, the probability of obtaining antigen-negative red blood cells (RBCs) for alloimmunized patients was calculated according to the estimated FY allele frequencies.

RESULTS:

The FY phenotyping and genotyping results were in 100% concordance. The allele frequencies of FY*A and FY*B in 500 central Thais were 0.962 (962/1,000) and 0.038 (38/1,000), respectively. Although the Fy(a-b-) phenotype was not observed in this study, FY*B(ES)/FY*B(ES) was identified by PCR-SSP in the Guinea family and was confirmed by DNA sequencing.

CONCLUSIONS:

Our results confirm the high frequency of the FY*A allele in the Thai population, similar to that of Asian populations. At least 500 Thai blood donors are needed to obtain two units of antigen-negative RBCs for the Fy(a-b+) phenotype.

• Authors: Oytip Nathalang, Kamphon Intharanut, Kanokpol Siriphanthong, Siriporn Nathalang, Pawinee Kupatawintu
• Journal: Annals of laboratory medicine (Ann Lab Med) Vol. 35 Issue 6 Pg. 618-23 (Nov 2015) ISSN: 2234-3814 [Electronic] Korea (South)
• PMID: 26354350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ACKR1 protein, human
• Duffy Blood-Group System
• Isoantibodies
• Receptors, Cell Surface
• DNA

Topics

• Adult
• Alleles
• Asian People (genetics)
• Base Sequence
• Blood Donors
• DNA (chemistry, genetics, metabolism)
• Duffy Blood-Group System (genetics, immunology)
• Female
• Gene Frequency
• Genotype
• Humans
• Isoantibodies (blood, immunology)
• Male
• Middle Aged
• Phenotype
• Polymerase Chain Reaction
• Receptors, Cell Surface (genetics, immunology)
• Sequence Analysis, DNA
• Thailand
• Young Adult