Abstract |
The development of aquaculture has been hampered by different aquatic pathogens that can cause edwardsiellosis, vibriosis, or other diseases. Therefore, developing a broad spectrum vaccine against different fish diseases is necessary. In this study, fructose 1,6-bisphosphate aldolase (FBA), a conserved enzyme in the glycolytic pathway, was demonstrated to be located in the non-cytoplasmic components of five aquatic pathogenic bacteria and exhibited remarkable protection and cross-protection against these pathogens in turbot and zebrafish. Further analysis revealed that sera sampled from vaccinated turbot had a high level of specific antibody and bactericidal activity against these pathogens. Meanwhile, the increased expressions of immune response-related genes associated with antigen recognition and presentation indicated that the adaptive immune response was effectively aroused. Taken together, our results suggest that FBA can be utilized as a broad-spectrum vaccine against various pathogenic bacteria of aquaculture in the future.
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Authors | Zhongyang Sun, Binbing Shen, Haizhen Wu, Xiangyu Zhou, Qiyao Wang, Jingfan Xiao, Yuanxing Zhang |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 46
Issue 2
Pg. 638-47
(Oct 2015)
ISSN: 1095-9947 [Electronic] England |
PMID | 26256425
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- Bacterial Proteins
- Bacterial Vaccines
- Fructose-Bisphosphate Aldolase
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Topics |
- Adaptive Immunity
- Animals
- Aquaculture
- Bacteria
(immunology)
- Bacterial Proteins
(genetics, immunology)
- Bacterial Vaccines
(genetics, immunology)
- Flatfishes
(immunology)
- Fructose-Bisphosphate Aldolase
(genetics, immunology)
- Immunity, Innate
- Sequence Analysis, DNA
(veterinary)
- Zebrafish
(immunology)
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