[Study on anti-inflammation effect and involved mechanism of Guizhi Fuling capsule and its active complex].

Abstract

The aim of this study was to investigate the anti-inflammatory effect of Guizhi Fuling capsule and its active complex (consistent of 15 active compounds) on LPS-induced RAW264. 7 cells. The effect of Guizhi Fuling capsule and its active complex on cell viability in RAW264. 7 cells were determined by MTT assay. The inhibitory effect of Guizhi Fuling capsule and active complex on the releasing of IL-1β, TNF-α and PGE2 induced by LPS in RAW264. 7 cells was detected by ELISA assay. The expression of IL-1β and mPGES-1 in Guizhi Fuling capsule or active complex treated RAW264. 7 cells was examined by Western blot assay. Guizhi Fuling capsule and active complex showed no significant effect on the cell viability in RAW264. 7 cells at doses range from 12.5 to 400 mg x L(-1). Compared with LPS treated group, Guizhi Fuling capsule and active complex dose dependently reduced the releasing of IL-1β, TNF-α and PGE2 induced by LPS in RAW264. 7 cells. Moreover, the expression of IL-1β and mPGES-1 was decreased after Guizhi Fuling capsule and active complex treatment, which might contribute to the inhibitory effect of Guizhi Fuling capsule in the releasing of IL-1β, TNF-α and PGE2. This study provided the evidence that Guizhi Fuling capsule and active complex remarkably inhibited the releasing of IL-1β, TNF-α and PGE2induced by LPS in RAW264. 7 cells by reducing the expression IL-1β and mPGES-1. This study provided an experimental basis of Guizhi Fuling capsule for the treatment of inflammation and a theoretical basis for the development of effective compounds of Guizhi Fuling capsule.

• Authors: Zhen-zhen Zhang, Xin-zhuang Zhang, Na Li, Liang Cao, Gang Ding, Zhen-zhong Wang, Wei Xiao
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 40 Issue 6 Pg. 993-8 (Mar 2015) ISSN: 1001-5302 [Print] China
• PMID: 26226733 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Drugs, Chinese Herbal
• Interleukin-1beta
• Tumor Necrosis Factor-alpha
• guizhi-fuling

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Line
• Cell Survival (drug effects)
• Drugs, Chinese Herbal (pharmacology)
• Inflammation (immunology)
• Interleukin-1beta (immunology)
• Macrophages (drug effects, immunology)
• Mice
• Tumor Necrosis Factor-alpha (immunology)