Abstract |
Previous studies have revealed several targets of miR-10b, such as syndecan-1, HOXD10, TBX5, and E-cadherin. In this study, we aimed to assess whether Krüppel-like factor 4 (KLF4) is a target gene of miR-10b in gastric cancer (GC). Targeting of KLF4 by miR-10b was confirmed by dual- luciferase reporter assays. The expression levels of miR-10b and KLF4 mRNA in 5 different gastric cancer cell lines and 65 pairs of gastric cancer tissues were detected by Real-time PCR. In addition, KLF4 protein in gastric cancer cell lines and 30 GC tissues was measured by western blotting and immunochemistry, respectively. KLF4 is a direct target gene of miR-10b in GC, and its expression is reduced by miR-10b at both mRNA and protein levels. In addition, the expression level of miR-10b was tendentiously upregulated in GC tissues while the expression levels of KLF4 mRNA and protein were decreased in gastric cancer tissues compared with normal adjacent tissue. There was a dramatically inverse correlation between the expression levels of miR-10b and KLF4 mRNA in GC (r=-0.339, P=0.006). These findings indicate that miR-10b was upregulated in GC and may have a key role in GC pathogenesis and development through the downregulation of its target gene KLF4.
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Authors | Zhihong Ma, Yingrong Chen, Lishan Min, Liqin Li, Huilian Huang, Jing Li, Qiang Yan, Pengtao Song, Lichen Dai, Xing Yao |
Journal | International journal of clinical and experimental pathology
(Int J Clin Exp Pathol)
Vol. 8
Issue 5
Pg. 5071-9
( 2015)
ISSN: 1936-2625 [Electronic] United States |
PMID | 26191201
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3' Untranslated Regions
- KLF4 protein, human
- Kruppel-Like Factor 4
- Kruppel-Like Transcription Factors
- MIRN10 microRNA, human
- MicroRNAs
- RNA, Messenger
- Luciferases
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Topics |
- 3' Untranslated Regions
- Binding Sites
- Carcinoma
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Down-Regulation
- Gene Expression Regulation, Neoplastic
- Genes, Reporter
- HEK293 Cells
- Humans
- Kruppel-Like Factor 4
- Kruppel-Like Transcription Factors
(genetics, metabolism)
- Luciferases
(genetics, metabolism)
- MicroRNAs
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Signal Transduction
- Stomach Neoplasms
(genetics, metabolism, pathology)
- Transfection
- Up-Regulation
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