Abstract | BACKGROUND: METHODS: We therefore investigated the genotoxicity of an aqueous extract of So-Cheong-Ryong-Tang (SCRT) in two in vitro and one in vivo assays: a bacterial reverse mutation assay (Ames test), a chromosomal aberration assay, and a micronucleus assay, respectively. RESULTS: In the bacterial reverse mutation assay, SCRT did not increase revertant colony numbers in Salmonella. typhimurium strains (TA98, TA100, TA1535, and TA1537) or an Escherichia coli strain (WP2 uvrA) regardless of metabolic activation or the duration of treatment. However, statistically significant differences in the incidence of chromosomal aberrations following SCRT>4000 μg/mL were observed in Chinese hamster lung cells exposed with or without an S9 enzyme and cofactor mixture. CONCLUSIONS: These results suggest further genotoxic testing of SCRT, such as a comet assay, to ascertain its generally recognized safety.
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Authors | Mee-Young Lee, Chang-Sebo Seo, Ji-Young Kim, Hyeun-Kyoo Shin |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 15
Pg. 235
(Jul 16 2015)
ISSN: 1472-6882 [Electronic] England |
PMID | 26178777
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- sho-seiryu-to
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Topics |
- Animals
- Chromosome Aberrations
- Cricetinae
- Cricetulus
- DNA Damage
- Drugs, Chinese Herbal
(adverse effects, therapeutic use)
- Escherichia coli
(genetics)
- Humans
- Lung
(drug effects)
- Magnoliopsida
- Medicine, Korean Traditional
- Micronucleus Tests
- Mutagenicity Tests
(methods)
- Mutation
- Phytotherapy
(adverse effects)
- Respiratory Tract Diseases
(drug therapy)
- Salmonella typhimurium
(genetics)
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