Relationship between genetic polymorphisms of DNA ligase 1 and non-small cell lung cancer susceptibility and radiosensitivity.

Abstract

The aim of this study was to examine the relationship between genetic polymorphisms in DNA ligase 1 (LIG1) and non-small cell lung cancer (NSCLC) susceptibility and radiosensitivity in a Chinese population. This was a case-control study that included 352 NSCLC patients and 448 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism analysis was conducted to detect HaeIII polymorphisms in exon 6 of the LIG1 gene in this popula-tion. This information was used to observe the effects of radiation in pa-tients with different genotypes in order to determine the genotypes as-sociated with radiosensitivity. The CC genotype and C allele frequency were significantly higher in the NSCLC group than in the control group (P = 0.012 and P = 0.023, respectively). The relative risk of experienc-ing NSCLC was 2.55 [95% confidence interval (CI), 1.12-3.98] for CC homozygous patients and 0.87 (95%CI, 0.46-1.88) for AA homozygous patients. Analysis of LIG1 genetic polymorphisms and radiosensitiv-ity of NSCLC patients showed that AA homozygous patients were sig-nificantly more radiosensitive than the control group (AA vs AC, P = 0.014; AA vs CC, P < 0.001; AC vs CC, P = 0.023). Therefore, the LIG1 CC genotype was associated with susceptibility to NSCLC, and the AA genotype demonstrated increased radiosensitivity compared to the AC and CC genotypes.

• Authors: H Tian, X He, L Yin, W J Guo, Y Y Xia, Z X Jiang
• Journal: Genetics and molecular research : GMR (Genet Mol Res) Vol. 14 Issue 2 Pg. 7047-52 (Jun 26 2015) ISSN: 1676-5680 [Electronic] Brazil
• PMID: 26125914 (Publication Type: Journal Article)

Chemical References

• LIG1 protein, human
• DNA Ligases
• DNA Ligase ATP

Topics

• Aged
• Alleles
• Carcinoma, Non-Small-Cell Lung (genetics, pathology, radiotherapy)
• Case-Control Studies
• DNA Ligase ATP
• DNA Ligases (genetics)
• Exons
• Female
• Gamma Rays (therapeutic use)
• Gene Expression
• Gene Frequency
• Genetic Predisposition to Disease
• Homozygote
• Humans
• Lung Neoplasms (genetics, pathology, radiotherapy)
• Male
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Radiation Tolerance