Central Administration of the Ciliary Neurotrophic Factor Analogue, Axokine, Does Not Play a Role in Long-Term Energy Homeostasis in Adult Mice.

Abstract

BACKGROUND/AIMS:

Ciliary neurotrophic factor (CNTF) exerts powerful anorectic effects and has been suggested to regulate long-term energy balance by inducing adult neurogenesis in the arcuate nucleus of the hypothalamus.

METHODS:

The CNTF analogue, Axokine, was infused into the lateral ventricle of high-fat-fed mice for 1 week. Food intake, energy expenditure, body mass, glucose metabolism, and neurogenesis in the arcuate nucleus (ARC) of the hypothalamus were assessed 3 weeks after cessation of Axokine treatment.

RESULTS:

Short-term administration of Axokine induced an anorexic response but did not promote sustained weight loss. Instead, a rapid rebound in food intake and body mass occurred immediately after cessation of Axokine treatment, and this tended to reduce insulin sensitivity. Immunolabeling of 5-bromo-2'-deoxyuridine revealed limited neurogenesis in the ARC 3 weeks after Axokine treatment.

CONCLUSION:

These findings suggest that Axokine/CNTF does not induce substantial or sustained ARC neurogenesis or contribute to the long-term regulation of energy balance in mice.

• Authors: Melissa L Borg, Alex Reichenbach, Moyra Lemus, Brian J Oldfield, Zane B Andrews, Matthew J Watt
• Journal: Neuroendocrinology (Neuroendocrinology) Vol. 103 Issue 3-4 Pg. 223-9 ( 2016) ISSN: 1423-0194 [Electronic] Switzerland
• PMID: 26088805 (Publication Type: Journal Article)
• Copyright: © 2015 S. Karger AG, Basel.

Chemical References

• Ciliary Neurotrophic Factor
• Insulin
• Neuropeptide Y
• Green Fluorescent Proteins
• axokine
• Glucose

Topics

• Analysis of Variance
• Animals
• Arcuate Nucleus of Hypothalamus (drug effects)
• Body Weight (drug effects)
• Ciliary Neurotrophic Factor (pharmacology)
• Eating (drug effects)
• Energy Metabolism (drug effects)
• Glucose (metabolism)
• Green Fluorescent Proteins (genetics, metabolism)
• Injections, Intraventricular
• Insulin (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Neurogenesis (drug effects)
• Neuropeptide Y (genetics, metabolism)
• Oxygen Consumption (drug effects)
• Time Factors