PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells.

Abstract

PURPOSE:

Sertoli-cell only syndrome is one of the reasons for male infertility but its pathogenesis remains unclear. PRPS2, a subset of PRS, is reported to be a potential protein associated with Sertoli-cell only syndrome. In this study we further investigated the correlation between PRPS2 and Sertoli-cell only syndrome, and evaluated the effect of PRPS2 expression on apoptosis of TM4 Sertoli cells.

MATERIALS AND METHODS:

PRPS2 expression was detected in patients with Sertoli-cell only syndrome and normal spermatogenesis, and in Sertoli-cell only syndrome mouse models by immunohistochemistry, quantitative reverse transcription-polymerase chain reaction and Western blot. PRPS2 expression in TM4 Sertoli cells was then down-regulated and up-regulated by lentivirus vectors. The effect of PRPS2 expression on cell apoptosis and cell cycle transition was evaluated by flow cytometry.

RESULTS:

PRPS2 expression in patients with Sertoli-cell only syndrome was significantly greater than in those with normal spermatogenesis. A significant increase in PRPS2 expression was observed in Sertoli-cell only syndrome mouse models. PRPS2 over expression significantly inhibited cell apoptosis and promoted cell cycle transition in TM4 Sertoli cells. However, PRPS2 down-regulation showed a reverse effect. Moreover, results revealed that PRPS2 over expression inhibited cell apoptosis via the p53/Bcl-2/caspase-9/caspase-3/caspase-6/caspase-7 signaling pathway.

CONCLUSIONS:

PRPS2 expression correlates with Sertoli-cell only syndrome and inhibits the apoptosis of TM4 Sertoli cells via the p53/Bcl-2/caspases signaling pathway.

• Authors: Bin Lei, Bo Wan, Jian Peng, Yu Yang, Daojun Lv, Xuming Zhou, Fangpeng Shu, Fei Li, Liren Zhong, Huayan Wu, Xiangming Mao
• Journal: The Journal of urology (J Urol) Vol. 194 Issue 5 Pg. 1491-7 (Nov 2015) ISSN: 1527-3792 [Electronic] United States
• PMID: 26004865 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

Chemical References

• RNA
• PRPS2 protein, human
• Ribose-Phosphate Pyrophosphokinase

Topics

• Adult
• Animals
• Apoptosis (genetics)
• Blotting, Western
• Cells, Cultured
• Disease Models, Animal
• Gene Expression Regulation
• Humans
• Male
• Mice
• Mice, Inbred C57BL
• Middle Aged
• RNA (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Ribose-Phosphate Pyrophosphokinase (biosynthesis, genetics)
• Sertoli Cell-Only Syndrome (genetics, metabolism, pathology)
• Sertoli Cells (metabolism, pathology)
• Young Adult