Platonin preserves blood-brain barrier integrity in septic rats.

Abstract

OBJECTIVES:

Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats.

METHODS:

A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring.

RESULTS:

Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA)-markers of systemic inflammation and oxidation-and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels-markers of BBB integrity-in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023).

CONCLUSION:

Platonin could attenuate sepsis-induced BBB integrity loss in rats.

• Authors: Chia-Tse Yeh, Ming-Chang Kao, Cay-Huyen Chen, Chun-Jen Huang
• Journal: Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists (Acta Anaesthesiol Taiwan) Vol. 53 Issue 1 Pg. 12-5 (Mar 2015) ISSN: 1875-452X [Electronic] China (Republic : 1949- )
• PMID: 25791838 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015. Published by Elsevier B.V.

Chemical References

• Claudin-5
• Cldn5 protein, rat
• Interleukin-6
• Thiazoles
• platonin

Topics

• Animals
• Blood-Brain Barrier (drug effects)
• Brain Edema (etiology)
• Claudin-5 (analysis)
• Interleukin-6 (blood)
• Male
• Oxidative Stress
• Rats
• Rats, Sprague-Dawley
• Sepsis (drug therapy, metabolism)
• Thiazoles (pharmacology, therapeutic use)