Vitamin d deficiency attenuates high-fat diet-induced hyperinsulinemia and hepatic lipid accumulation in male mice.

Abstract

It is increasingly recognized that vitamin D deficiency is associated with increased risks of metabolic disorders among overweight children. A recent study showed that vitamin D deficiency exacerbated inflammation in nonalcoholic fatty liver disease through activating toll-like receptor 4 in a high-fat diet (HFD) rat model. The present study aimed to further investigate the effects of vitamin D deficiency on HFD-induced insulin resistance and hepatic lipid accumulation. Male ICR mice (35 d old) were randomly assigned into 4 groups as follows. In control diet and vitamin D deficiency diet (VDD) groups, mice were fed with purified diets. In HFD and VDD+HFD groups, mice were fed with HFD. In VDD and VDD+HFD groups, vitamin D in feed was depleted. Feeding mice with vitamin D deficiency diet did not induce obesity, insulin resistance, and hepatic lipid accumulation. By contrary, vitamin D deficiency markedly alleviated HFD-induced overweight, hyperinsulinemia, and hepatic lipid accumulation. Moreover, vitamin D deficiency significantly attenuated HFD-induced up-regulation of hepatic peroxisome proliferator-activated receptor γ, which promoted hepatic lipid uptake and lipid droplet formation, and its target gene cluster of differentiation 36. In addition, vitamin D deficiency up-regulated carnitine palmitoyltrans 2, the key enzyme for fatty acid β-oxidation, and uncoupling protein 3, which separated oxidative phosphorylation from ATP production, in adipose tissue. These data suggest that vitamin D deficiency is not a direct risk factor for obesity, insulin resistance, and hepatic lipid accumulation. Vitamin D deficiency alleviates HFD-induced overweight, hyperinsulinemia, and hepatic lipid accumulation through promoting fatty acid β-oxidation and elevating energy expenditure in adipose tissue.

• Authors: Xiao-Jing Liu, Bi-Wei Wang, Cheng Zhang, Mi-Zhen Xia, Yuan-Hua Chen, Chun-Qiu Hu, Hua Wang, Xi Chen, De-Xiang Xu
• Journal: Endocrinology (Endocrinology) Vol. 156 Issue 6 Pg. 2103-13 (Jun 2015) ISSN: 1945-7170 [Electronic] United States
• PMID: 25774554 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Body Weight (drug effects)
• Diet, High-Fat (adverse effects)
• Eating (drug effects)
• Energy Intake (drug effects)
• Hyperinsulinism (etiology)
• Insulin Resistance (physiology)
• Liver (drug effects, metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Obesity (metabolism)
• Real-Time Polymerase Chain Reaction
• Vitamin D Deficiency (metabolism, physiopathology)