Saponins, especially platyconic acid A, from Platycodon grandiflorum reduce airway inflammation in ovalbumin-induced mice and PMA-exposed A549 cells.

Abstract

We investigated the inhibitory effects of Platycodon grandiflorum root-derived saponins (Changkil saponins: CKS) on ovalbumin-induced airway inflammation in mice. CKS suppressed leukocytes number, IgE, Th1/Th2 cytokines, and MCP-1 chemokine secretion in bronchoalveolar lavage fluid. Also, ovalbumin-increased MUC5AC, MMP-2/9, and TIMP-1/-2 mRNA expression, NF-κB activation, leukocytes recruitment, and mucus secretion were inhibited by CKS treatment. Moreover, the active component of CKS, platyconic acid A (PA), suppressed PMA-induced MUC5AC mRNA expression (from 2.1 ± 0.2 to 1.1 ± 0.1) by inhibiting NF-κB activation (from 2.3 ± 0.2 to 1.2 ± 0.1) via Akt (from 3.7 ± 0.3 to 2.1 ± 0.2) (p < 0.01) in A549 cells. Therefore, we demonstrate that CKS or PA suppressed the development of respiratory inflammation, hyperresponsiveness, and remodeling by reducing allergic responses, and they may be potential herbal drugs for allergen-induced respiratory disease prevention.

• Authors: Jae Ho Choi, Sun Woo Jin, Hyung Gyun Kim, Chul Yung Choi, Hyun Sun Lee, Shi Yong Ryu, Young Chul Chung, Young Jung Hwang, Yeon Ji Um, Tae Cheon Jeong, Hye Gwang Jeong
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 63 Issue 5 Pg. 1468-76 (Feb 11 2015) ISSN: 1520-5118 [Electronic] United States
• PMID: 25590691 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Muc5ac protein, mouse
• Mucin 5AC
• Saponins
• Tissue Inhibitor of Metalloproteinase-1
• Triterpenes
• platyconic acid
• Ovalbumin
• Matrix Metalloproteinase 2
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage)
• Cell Line
• Cytokines (genetics, immunology)
• Female
• Humans
• Lung (drug effects, immunology)
• Matrix Metalloproteinase 2 (genetics, immunology)
• Mice
• Mice, Inbred ICR
• Mucin 5AC (genetics, immunology)
• Ovalbumin (adverse effects)
• Plant Roots (chemistry)
• Platycodon (chemistry)
• Saponins (administration & dosage)
• Tetradecanoylphorbol Acetate (adverse effects)
• Tissue Inhibitor of Metalloproteinase-1 (genetics, immunology)
• Triterpenes (administration & dosage)