Osteogenesis imperfecta (OI) is a heterogeneous group of inheritable connective tissue disorders characterized by mutation in genes involved in
collagen synthesis and leading to increased bone fragility, low bone mass, impaired bone material properties and abnormally high bone matrix mineralization. Recessive
OI type VI is caused by mutation in SERPINF1 leading to a loss-of-function of
pigment epithelium-derived factor (PEDF) a
collagen-
binding protein with potent antiangiogenic activity. Affected patients develop a severe OI phenotype with a striking histological characteristic, rare in other OI types, of an excess of osteoid tissue and prolonged mineralization lag time. To get insights into matrix mineralization, we evaluated biopsies from 9 affected children by quantitative and by high-resolution backscattered electron imaging and assessed bone mineralization density distribution. Thickness, shape and arrangement of
mineral particles were measured in a subset of 4 patients by
synchrotron small angle X-ray scattering. Typical
calcium content in the bone matrix was found to be increased compared to controls, even exceeding values found previously in OI patients with
collagen-gene mutations. A main characteristic however, is the coexistence of this highly mineralized bone matrix with seams showing abnormally low
mineral content. Atypical
collagen fibril organization was found in the perilacunar region of young osteocytes, suggesting a disturbance in the early steps of mineralization. These observations are consistent with the presence of a heterogeneous population of
mineral particles with unusual size, shape and arrangement, especially in the region with lower
mineral content. The majority of the particles in the highly mineralized bone areas were less disorganized, but smaller and more densely packed than in controls and in previously measured OI patients. These data suggest that the lack of PEDF impairs a proper osteoblast-osteocyte transition and consequently affects the early steps of mineralization, downstream
collagen assembly making
OI type VI different from "classical" OI with mutations in
collagen-type I encoding genes, despite the typical hypermineralization of the bone matrix.