Sinapic acid protects heart against ischemia/reperfusion injury and H9c2 cardiomyoblast cells against oxidative stress.

Abstract

The present study was designed to evaluate antioxidant and cardioprotective potential of sinapic acid (SA) against ischemia/reperfusion (I/R) injury. Cardiac functional recovery after I/R was evaluated by percentage rate pressure product (%RPP) and percentage coronary flow (%CF). Myocardial injury was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining and LDH enzyme leakage. Oxidative stress was estimated by lipid peroxidation level. eNOS protein expression in reperfused heart was assessed using Western blot method. Finally, in order to support the antioxidant effect of SA, in vitro protective potential of SA was assessed on H2O2-induced oxidative stress in H9c2 cardiomyoblast cells. The overall results demonstrated that I/R induced cardiac dysfunction, injury and oxidative stress was attenuated by SA treatment. Moreover, in vitro results also shown that, SA protects H9c2 cells from oxidative stress and modulates mitochondrial membrane permeability transition (MPT). In conclusion, coupled results from both in vivo and in vitro experiments have confirmed that SA with antioxidant role protects cardiac cells and its functions from I/R induced oxidative stress.

• Authors: Thangarasu Silambarasan, Jeganathan Manivannan, Mani Krishna Priya, Natarajan Suganya, Suvro Chatterjee, Boobalan Raja
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 456 Issue 4 Pg. 853-9 (Jan 24 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 25511706 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Cardiotonic Agents
• Coumaric Acids
• Mitochondrial Membrane Transport Proteins
• Mitochondrial Permeability Transition Pore
• sinapinic acid
• Hydrogen Peroxide
• L-Lactate Dehydrogenase
• Nitric Oxide Synthase Type III

Topics

• Animals
• Cardiotonic Agents (pharmacology, therapeutic use)
• Coumaric Acids (pharmacology, therapeutic use)
• Hydrogen Peroxide
• In Vitro Techniques
• L-Lactate Dehydrogenase (metabolism)
• Lipid Peroxidation (drug effects)
• Male
• Mitochondria, Heart (drug effects, enzymology)
• Mitochondrial Membrane Transport Proteins (metabolism)
• Mitochondrial Permeability Transition Pore
• Myoblasts (drug effects, metabolism, pathology)
• Myocardial Infarction (complications, pathology, physiopathology)
• Myocardial Reperfusion Injury (drug therapy, pathology, physiopathology, prevention & control)
• Myocytes, Cardiac (drug effects, metabolism, pathology)
• Nitric Oxide Synthase Type III (metabolism)
• Oxidative Stress (drug effects)
• Rats, Wistar
• Recovery of Function (drug effects)