Arterial tissue and plasma concentration of enzymatic-driven oxysterols are associated with severe peripheral atherosclerotic disease and systemic inflammatory activity.

Abstract

INTRODUCTION:

Cholesterol undergoes oxidation via both enzymatic stress- and free radical-mediated mechanisms, generating a wide range of oxysterols. In contrast to oxidative stress-driven metabolites, enzymatic stress-derived oxysterols are scarcely studied in their association with atherosclerotic disease in humans.

METHODS:

24S-hydroxycholesterol (24S-HC), 25-hydroxycholesterol (25-HC), and 27-hydroxycholesterol (27-HC) were assessed in plasma and arteries with atherosclerotic plaques from 10 patients (54-84 years) with severe peripheral artery disease (PAD) as well as arteries free of atherosclerotic plaques from 13 individuals (45-78 years, controls).

RESULTS:

Plasma 25-HC was higher in PAD individuals than in controls (6.3[2] vs. 3.9[1.9] ng/mgCol; p = 0.004). 24S-HC and 27-HC levels were, respectively, five- and 20-fold higher in the arterial tissue of PAD individuals than in those of the controls (p = 0.016 and p = 0.001). Plasma C-reactive protein correlated with plasma 24-HC (r = 0.51; p = 0.010), 25-HC (r = 0.75; p < 0.001), 27-HC (r = 0.48; p = 0.015), and with tissue 24S-HC (r = 0.4; p = 0.041) and 27-HC (r = 0.46; p = 0.023).

CONCLUSION:

Arterial intima accumulation of 27-HC and 24S-HC is associated with advanced atherosclerotic disease and systemic inflammatory activity in individuals with severe PAD.

• Authors: V W M Virginio, V S Nunes, F A Moura, F H Menezes, N A Andreollo, F Rogerio, D Z Scherrer, E C R Quintão, E Nakandakare, O Petrucci, W Nadruz-Junior, E C de Faria, A C Sposito
• Journal: Free radical research (Free Radic Res) Vol. 49 Issue 2 Pg. 199-203 (Feb 2015) ISSN: 1029-2470 [Electronic] England
• PMID: 25465091 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydroxycholesterols
• 24-hydroxycholesterol
• 27-hydroxycholesterol
• 25-hydroxycholesterol
• C-Reactive Protein

Topics

• Aged
• Aged, 80 and over
• Arteries (chemistry)
• C-Reactive Protein (analysis)
• Female
• Humans
• Hydroxycholesterols (blood)
• Inflammation (blood)
• Male
• Middle Aged
• Peripheral Arterial Disease (blood)