This study investigated the effects of hybridized micro and nano structured
collagen implants on tendon healing in an experimental
tendon injury in rabbits. Fifty mature male New Zealand white rabbits were randomly divided into two groups of treated and control. Two cm of the left Achilles tendon were discarded. In the treated group, a 3-dimensional (3D)
collagen implant was engineered and implanted in the defect area. No implant was used in the control group. At day 120 after injury, the Achilles tendon of the animals were ultrasonographically (days 0-120 after injury) and radiographically (day 120 after injury) examined, and the animals were euthanized. The tendons were dissected and used for gross pathological, histopathological, ultra-structural and biomechanical investigations. Application of the
collagen implant significantly increased the diameter of the newly regenerated tissue in the defect area compared to the control tendons. Treatment also significantly increased the echogenicity and homogeneity of the injured area, the diameter of the
collagen fibrils and fibers, maturity of the tenoblasts, number of tenocytes,
collagen density, alignment, ultimate and yield load, stiffness, stress and modulus of elasticity. The
collagen implants were almost totally absorbed 120 days after surgery. No inflammatory reaction or tissue degeneration or
necrosis was evident in the treated tendons compared to the control ones. 3D
collagen implants produced a newly regenerated tendinous tissue at the defect area that was morphologically and biomechanically superior to the control group. This
collagen implant was biocompatible and biodegradable with high bio-safety in rabbits.