Pseudogene CYP4Z2P 3'UTR promotes angiogenesis in breast cancer.

Abstract

Pseudogenes have long been marked as "false" genes, which are similar with real genes but have no apparent function. The 3'UTR is well-known to regulate gene expression post-transcriptionally. Our recent evidence, however, indicates novel functional roles of pseudogene CYP4Z2P 3'UTR (Z2P-UTR). We found that ectopic expression of Z2P-UTR in breast cancer cells significantly increased the expression of VEGF-A without affecting cell proliferation in vitro. Meanwhile, conditioned medium (CM) from Z2P-UTR overexpression cells enhanced proliferation, migration and tube formation of HUVEC, and promoted angiogenesis in ex vivo models. Also, CM increased the expression of VEGFR2 in HUVEC. Our data suggest that Z2P-UTR can promote breast cancer angiogenesis partly via paracrine pathway of VEGF-A/VEGFR2.

• Authors: Lufeng Zheng, Xiaoman Li, Yi Gu, Yihua Ma, Tao Xi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 453 Issue 3 Pg. 545-51 (Oct 24 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 25281903 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• 3' Untranslated Regions
• DNA Primers
• RNA, Messenger
• Cytochrome P-450 Enzyme System

Topics

• 3' Untranslated Regions
• Animals
• Base Sequence
• Blotting, Western
• Breast Neoplasms (blood supply, metabolism, pathology)
• Cell Line
• Cell Line, Tumor
• Chick Embryo
• Cytochrome P-450 Enzyme System (genetics)
• DNA Primers
• Female
• Humans
• Neovascularization, Pathologic (genetics)
• Nucleic Acid Conformation
• Pseudogenes
• RNA, Messenger (chemistry, genetics)
• Rats
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction