Abstract | OBJECTIVE: METHOD: RESULTS: Combination treatment significantly reduced infarct volume of cerebral ischemic regions compared with treatment of rt-PA and TSD alone and that of the saline control group (P<0.01). A combined treatment of rt-PA (4 mg/kg) with TSD [0.7 g/(kg·day)] significantly increased cerebral blood flow in a time (100 and 120 min) dependent manner (P<0.05). Interestingly, despite treatment of rt-PA (4 mg/kg) alone significantly reduced the expressions of HIF-1α, TNF-α, and iNOS in ischemic regions, reduction of these expressions were more potentiated when combined with TSD (P<0.05). Combination treatment also reduced apoptosis as measured by a significant reduction in active caspase-3 expression in the ischemic brain compared with the MCAO group (P<0.01). CONCLUSIONS: A combination of low-dose rt-PA and TSD after embolic stroke reduced infarct volume, improved cerebral blood flow and provided neuroprotection and these effects were associated with reduction of apoptosis and attenuation of HIF-1α, TNF-α and iNOS expression. These results provide a positive contribution to better understand the therapeutic value of the combination of TSD with rt-PA in ischemic stroke and may support further clinical evaluation.
|
Authors | Ting-Lin Yen, Eng-Thiam Ong, Kuan-Hung Lin, Chao-Chien Chang, Thanasekaran Jayakumar, Song-Chow Lin, Tsorng-Han Fong, Joen-Rong Sheu |
Journal | Chinese journal of integrative medicine
(Chin J Integr Med)
Pg.
(Sep 24 2014)
ISSN: 1672-0415 [Print] China |
PMID | 25253548
(Publication Type: Journal Article)
|