[Apoptosis of melanoma B16 cells induced by gambogenic acid].

Abstract

OBJECTIVE:

To study the inhibitory effect of Gambogenic acid (GNA) on melanoma B16 cells proliferation, and to explore the role of cell apoptosis.

METHODS:

The inhibitory effect of GNA on the proliferation of B16 cells was measured by methyl thiazolyl tetrazolium (MTT) assay; Alternation of B16 cells ultrastructure was detected by AO/EB staining under fluorescent microscope; Flow cytometry was used to detect intracellular reactive oxygen species (ROS) in B16 cells generated by GNA treatment Western blotting was used to investigate the expression of intracellular Caspase-3 proteins changes.

RESULTS:

MTT results showed that the GNA within a certain time and a certain concentration significantly suppressed the proliferation of B16 cells and morphological changes were observed by fluorescence microscope on B16 cells after GNA treatment. AO/EB staining showed that the major cell density decreased. GNA treated cells showed obvious apoptotic status. After the cells treated with GNA, in a short period of time, intracellular ROS levels increased dramatically compared with the control group (P < 0.01), and the mitochondrial membrane had a low potential consistently. Western blotting results showed that changes of intracellular proteins expression in the release of Caspase-3 proteins expression levels were increased after GNA treatment.

CONCLUSION:

GNA can inhibit malignant melanoma B16 cells growth and proliferation and induce apoptosis within a certain time and at a certain concentration.

• Authors: Yong-Jin Tan, Xuan Zhang, Hui Cheng, Qing-Lin Li
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 37 Issue 3 Pg. 469-73 (Mar 2014) ISSN: 1001-4454 [Print] China
• PMID: 25174115 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Reactive Oxygen Species
• Terpenes
• Xanthenes
• neo-gambogic acid
• Casp3 protein, mouse
• Caspase 3

Topics

• Animals
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Flow Cytometry
• Garcinia (chemistry)
• Melanoma, Experimental (metabolism, pathology)
• Mice
• Mitochondria (drug effects, metabolism)
• Reactive Oxygen Species (metabolism)
• Terpenes (pharmacology)
• Xanthenes (pharmacology)