Abstract | OBJECTIVE: MATERIALS AND METHODS: PC12 cells were cultivated for 8 h in GSD condition in both the absence and presence of curcumin, linalool, rutin, safranal, thymoquinone, or combinations of them. At the end of the experiment, the cell viability was determined using MTT assay. RESULTS: The cells cultured in GSD condition showed a significant decrease in viability (28±1%) as compared with those cultured in standard condition (100±2%). In the presence of curcumin (10 µg/ml), linalool (16 µg/ml), rutin (200 µg/ml), safranal (50 µg/ml), and thymoquinone (1 µg/ml), the cell viability increased to 69±3.4% (p<0.001), 44±1.4% (p<0.01), 64±0.5% (p<0.001), 49±2% (p<0.001), and 70±3.2% (p<0.001), respectively. When different combinations of the agents were tested, the best cytoprotective activity was obtained from safranal + curcumin + thymoquinone (97±5%, p<0.01 vs. untreated cells). CONCLUSIONS:
|
Authors | Bagher Alinejad, Ahmad Ghorbani, Hamid Reza Sadeghnia |
Journal | Avicenna journal of phytomedicine
(Avicenna J Phytomed)
Vol. 3
Issue 4
Pg. 321-8
( 2013)
ISSN: 2228-7930 [Print] Iran |
PMID | 25050289
(Publication Type: Journal Article)
|