Fucosylated chondroitin sulfate from sea cucumber improves insulin sensitivity via activation of PI3K/PKB pathway.

Abstract

This study was to investigate the effects of fucosylated chondroitin sulfate (CHS) from sea cucumber on insulin sensitivity in skeletal muscle of type 2 diabetic mice induced by a high-fat high-sucrose diet (HFSD). CHS supplementation for 19 wk significantly improved insulin sensitivity by 20%, and reduced blood glucose and insulin levels. Western blotting assay showed that CHS significantly increased insulin-stimulated glucose transporter 4 (GLUT4) translocation to 1.7-fold, phosphorylation of phosphoinositide 3-kinase (PI3K) at p85 to 5.0-fold, protein kinase B (PKB) at Ser473 to 1.5-fold, and Thr308 to 1.6-fold in skeletal muscle. However, PI3K, PKB, and GLUT4 total proteins expression were unchangeable. In addition, qRT-PCR analysis proved that the insulin signaling was activated by CHS treatment, showing the increased mRNA expressions of glucose uptake-related key genes. It indicated that CHS improved insulin sensitivity by activation of PI3K/PKB signaling in skeletal muscle of type 2 diabetic mice. Identification of potential mechanism by which CHS increased insulin sensitivity might provide a new functional food or pharmaceutical application of sea cucumber.

• Authors: Shiwei Hu, Yaoguang Chang, Min He, Jingfeng Wang, Yuming Wang, Changhu Xue
• Journal: Journal of food science (J Food Sci) Vol. 79 Issue 7 Pg. H1424-9 (Jul 2014) ISSN: 1750-3841 [Electronic] United States
• PMID: 25041539 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

Chemical References

• Blood Glucose
• Glucose Transporter Type 4
• Insulin
• fucosylated chondroitin sulfate
• Chondroitin Sulfates
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Blood Glucose
• Chondroitin Sulfates (chemistry, pharmacology)
• Diabetes Mellitus, Experimental (metabolism)
• Glucose Transporter Type 4 (metabolism)
• Hyperinsulinism
• Insulin (blood)
• Insulin Resistance (physiology)
• Male
• Mice
• Mice, Inbred C57BL
• Muscle, Skeletal (metabolism)
• Phosphatidylinositol 3-Kinases (genetics, metabolism)
• Phosphorylation (drug effects)
• Proto-Oncogene Proteins c-akt (genetics, metabolism)
• Sea Cucumbers
• Signal Transduction (drug effects)