Abstract | BACKGROUND: METHOD: Three-day-old guinea pigs received parenteral solutions containing 0, 20, 60 or 180 µM ascorbylperoxide in the presence or not of 350 µM H2O2 (concentrations similar to those measured in parenteral nutrition). After 4 days, the lungs were collected for determination of glutathione's redox potential, caspase-3 activation (an apoptosis marker), alveolarization index (by histology), activation of Nrf2 and NF?B ( biological markers of oxidative stress), and IL-6 and PGJ2 levels (markers of NF?B activation). Groups were compared by ANOVA, p < 0.05. RESULTS: Loss of alveoli was associated with ascorbylperoxide in a dose-dependent manner, without an influence of H2O2. The dose-dependent activation of caspase-3 by ascorbylperoxide was lower in the presence of H2O2. Ascorbylperoxide induced an increase of redox potential in a dose-dependent manner, which reached a plateau in presence of H2O2. Nrf2 and NF?B were activated by H2O2 but not by ascorbylperoxide. CONCLUSION:
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Authors | Wesam Elremaly, Ibrahim Mohamed, Tiphaine Mialet-Marty, Thérèse Rouleau, Jean-Claude Lavoie |
Journal | Redox biology
(Redox Biol)
Vol. 2
Pg. 725-31
( 2014)
ISSN: 2213-2317 [Print] Netherlands |
PMID | 25009773
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-6
- NF-E2-Related Factor 2
- NF-kappa B
- Peroxides
- ascorbylperoxide
- 9-deoxy-delta-9-prostaglandin D2
- Hydrogen Peroxide
- Caspase 3
- Glutathione
- Ascorbic Acid
- Prostaglandin D2
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Topics |
- Animals
- Animals, Newborn
- Ascorbic Acid
(analogs & derivatives, toxicity)
- Caspase 3
(metabolism)
- Glutathione
(metabolism)
- Guinea Pigs
- Hydrogen Peroxide
(toxicity)
- Interleukin-6
(metabolism)
- Lung
(drug effects, metabolism)
- NF-E2-Related Factor 2
(metabolism)
- NF-kappa B
(metabolism)
- Oxidation-Reduction
(drug effects)
- Oxidative Stress
(drug effects)
- Parenteral Nutrition
- Peroxides
(toxicity)
- Prostaglandin D2
(analogs & derivatives, analysis)
- Pulmonary Alveoli
(physiopathology)
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